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We investigate early molecular events that might play a role in skeletal muscle remodeling during mechanical unloading (disuse). The effects of acute (6\u201312 h) hindlimb suspension on the soleus muscles from adult rats were examined. The integrity of plasma membrane lipid rafts was tested utilizing cholera toxin B subunit or fluorescent sterols. In addition, resting intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    level was analyzed. Acute disuse disturbed the plasma membrane lipid-ordered phase throughout the sarcolemma and was more pronounced in junctional membrane regions. Ouabain (1 \u00b5M), which specifically inhibits the Na-K-ATPase \u03b12 isozyme in rodent skeletal muscles, produced similar lipid raft changes in control muscles but was ineffective in suspended muscles, which showed an initial loss of \u03b12 Na-K-ATPase activity. Lipid rafts were able to recover with cholesterol supplementation, suggesting that disturbance results from cholesterol loss. Repetitive nerve stimulation also restores lipid rafts, specifically in the junctional sarcolemma region. Disuse locally lowered the resting intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration only near the neuromuscular junction of muscle fibers. Our results provide evidence to suggest that the ordering of lipid rafts strongly depends on motor nerve input and may involve interactions with the \u03b12 Na-K-ATPase. Lipid raft disturbance, accompanied by intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    dysregulation, is among the earliest remodeling events induced by skeletal muscle disuse.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00365.2016","type":"journal-article","created":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T21:20:34Z","timestamp":1489008034000},"page":"C627-C637","source":"Crossref","is-referenced-by-count":62,"title":["Membrane lipid rafts are disturbed in the response of rat skeletal muscle to short-term disuse"],"prefix":"10.1152","volume":"312","author":[{"given":"Alexey M.","family":"Petrov","sequence":"first","affiliation":[{"name":"Department of Normal Physiology, Kazan State Medical University, Kazan, Russia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violetta V.","family":"Kravtsova","sequence":"additional","affiliation":[{"name":"Department of General Physiology, St. Petersburg State University, 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