{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T18:40:05Z","timestamp":1718649605540},"reference-count":31,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p> Ethanol strongly augments secretin-stimulated, but not acetylcholine (ACh)-stimulated, fluid secretion from pancreatic duct cells. To understand its mechanism of action, we examined the effect of short-chain n-alcohols on fluid secretion and intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in guinea pig pancreatic ducts. Fluid secretion was measured by monitoring the luminal volume of isolated interlobular ducts. [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> was estimated using fura-2 microfluorometry. Methanol and ethanol at 0.3\u201310 mM concentrations significantly augmented fluid secretion and induced a transient elevation of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in secretin- or dibutyryl adenosine 3\u2032,5\u2032-cyclic monophosphate (DBcAMP)-stimulated ducts. However, they failed to affect fluid secretion and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in unstimulated and ACh-stimulated ducts. In contrast, propanol and butanol at 0.3\u201310 mM concentrations significantly reduced fluid secretion and decreased [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in unstimulated ducts and in ducts stimulated with secretin, DBcAMP, or ACh. Both stimulatory and inhibitory effects of n-alcohols completely disappeared after their removal from the perfusate. Propanol and butanol inhibited the plateau phase, but not the initial peak, of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> response to ACh as well as the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> elevation induced by thapsigargin, suggesting that they inhibit Ca<jats:sup>2+<\/jats:sup> influx. Removal of extracellular Ca<jats:sup>2+<\/jats:sup> reduced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in duct cells and completely abolished secretin-stimulated fluid secretion. In conclusion, there is a distinct cutoff point between ethanol (C2) and propanol (C3) in their effects on fluid secretion and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in duct cells. Short-chain n-alcohols appear to affect pancreatic ductal fluid secretion by activating or inhibiting the plasma membrane Ca<jats:sup>2+<\/jats:sup> channel. <\/jats:p>","DOI":"10.1152\/ajpcell.00373.2004","type":"journal-article","created":{"date-parts":[[2005,1,20]],"date-time":"2005-01-20T01:34:28Z","timestamp":1106184868000},"page":"C1431-C1439","source":"Crossref","is-referenced-by-count":6,"title":["Dual effects of <i>n<\/i>-alcohols on fluid secretion from guinea pig pancreatic ducts"],"prefix":"10.1152","volume":"288","author":[{"given":"Hiroyuki","family":"Hamada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Ishiguro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiko","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Shimano-Futakuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru B. 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