{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:37:39Z","timestamp":1792679859130,"version":"4.5.0"},"reference-count":45,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:p> This study examines whether fluid pressure (FP) modulates the L-type Ca<jats:sup>2+<\/jats:sup> channel in cardiomyocytes and investigates the underlying cellular mechanism(s) involved. A flow of pressurized (\u223c16 dyn\/cm<jats:sup>2<\/jats:sup>) fluid, identical to that bathing the myocytes, was applied onto single rat ventricular myocytes using a microperfusion method. The Ca<jats:sup>2+<\/jats:sup> current ( I<jats:sub>Ca<\/jats:sub>) and cytosolic Ca<jats:sup>2+<\/jats:sup> signals were measured using a whole cell patch-clamp and confocal imaging, respectively. It was found that the FP reversibly suppressed I<jats:sub>Ca<\/jats:sub> (by 25%) without altering the current-voltage relationships, and it accelerated the inactivation of I<jats:sub>Ca<\/jats:sub>. The level of I<jats:sub>Ca<\/jats:sub> suppression by FP depended on the level and duration of pressure. The Ba<jats:sup>2+<\/jats:sup> current through the Ca<jats:sup>2+<\/jats:sup> channel was only slightly decreased by the FP (5%), suggesting an indirect inhibition of the Ca<jats:sup>2+<\/jats:sup> channel during FP stimulation. The cytosolic Ca<jats:sup>2+<\/jats:sup> transients and the basal Ca<jats:sup>2+<\/jats:sup> in field-stimulated ventricular myocytes were significantly increased by the FP. The effects of the FP on the I<jats:sub>Ca<\/jats:sub> and on the Ca<jats:sup>2+<\/jats:sup> transient were resistant to the stretch-activated channel inhibitors, GsMTx-4 and streptomycin. Dialysis of myocytes with high concentrations of BAPTA, the Ca<jats:sup>2+<\/jats:sup> buffer, eliminated the FP-induced acceleration of I<jats:sub>Ca<\/jats:sub> inactivation and reduced the inhibitory effect of the FP on I<jats:sub>Ca<\/jats:sub> by \u224880%. Ryanodine and thapsigargin, abolishing sarcoplasmic reticulum Ca<jats:sup>2+<\/jats:sup> release, eliminated the accelerating effect of FP on the I<jats:sub>Ca<\/jats:sub> inactivation, and they reduced the inhibitory effect of FP on the I<jats:sub>Ca<\/jats:sub>. These results suggest that the fluid pressure indirectly suppresses the Ca<jats:sup>2+<\/jats:sup> channel by enhancing the Ca<jats:sup>2+<\/jats:sup>-induced intracellular Ca<jats:sup>2+<\/jats:sup> release in rat ventricular myocytes. <\/jats:p>","DOI":"10.1152\/ajpcell.00381.2007","type":"journal-article","created":{"date-parts":[[2008,2,14]],"date-time":"2008-02-14T02:25:23Z","timestamp":1202955923000},"page":"C966-C976","source":"Crossref","is-referenced-by-count":26,"title":["Fluid pressure modulates L-type Ca<sup>2+<\/sup> channel via enhancement of Ca<sup>2+<\/sup>-induced Ca<sup>2+<\/sup> release in rat ventricular myocytes"],"prefix":"10.1152","volume":"294","author":[{"given":"Sunwoo","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joon-Chul","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Jeong","family":"Son","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun-Hee","family":"Woo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Adachi-Akahane S, Cleemann L, Morad M. 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