{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:44:45Z","timestamp":1791020685166,"version":"4.1.0"},"reference-count":52,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,4,1]]},"abstract":"<jats:p> HCO[Formula: see text]-dependent fluid secretion by the corneal endothelium controls corneal hydration and maintains corneal transparency. Recently, it has been shown that mRNA for the cystic fibrosis transmembrane conductance regulator (CFTR) is expressed in the corneal endothelium; however, protein expression, functional localization, and a possible role in HCO[Formula: see text]transport have not been reported. Immunoblotting for CFTR showed a single band at \u223c170 kDa for both freshly isolated and primary cultures of bovine corneal endothelial cells. Indirect immunofluorescence confocal microscopy indicated that CFTR locates to the apical membrane. Relative changes in apical and basolateral chloride permeability were estimated by measuring the rate of fluorescence quenching of the halide-sensitive indicator 6-methoxy- N-ethylquinolinium iodide during Cl<jats:sup>\u2212<\/jats:sup>influx in the absence and presence of forskolin (FSK). Apical and basolateral Cl<jats:sup>\u2212<\/jats:sup> permeability increased 10- and 3-fold, respectively, in the presence of 50 \u03bcM FSK. FSK-activated apical chloride permeability was unaffected by H<jats:sub>2<\/jats:sub>DIDs (250 \u03bcM); however, 5-nitro-2-(3-phenylpropyl-amino)benzoic acid (NPPB; 50 \u03bcM) and glibenclamide (100 \u03bcM) inhibited activated Cl<jats:sup>\u2212<\/jats:sup>fluxes by 45% and 30%, respectively. FSK-activated basolateral Cl<jats:sup>\u2212<\/jats:sup> permeability was insensitive to NPPB, glibenclamide, or furosemide but was inhibited 80% by H<jats:sub>2<\/jats:sub>DIDS. HCO[Formula: see text] permeability was estimated by measuring changes in intracellular pH in response to quickly lowering bath [HCO[Formula: see text]]. FSK (50 \u03bcM) increased apical HCO[Formula: see text] permeability by twofold, which was inhibited 42% by NPPB and 65% by glibenclamide. Basolateral HCO[Formula: see text] permeability was unaffected by FSK. Genistein (50 \u03bcM) significantly increased apical HCO[Formula: see text] and Cl<jats:sup>\u2212<\/jats:sup> permeability by 1.8- and 16-fold, respectively. When 50 \u03bcM genistein was combined with 50 \u03bcM FSK, there was no further increase in Cl<jats:sup>\u2212<\/jats:sup> permeability; however, HCO[Formula: see text] permeability was reduced to the control level. In summary, we conclude that CFTR is present in the apical membrane of bovine corneal endothelium and could contribute to transendothelial Cl<jats:sup>\u2212<\/jats:sup> and HCO[Formula: see text] transport. Furthermore, there is a cAMP-activated Cl<jats:sup>\u2212<\/jats:sup> pathway on the basolateral membrane that is not CFTR. <\/jats:p>","DOI":"10.1152\/ajpcell.00384.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:07:47Z","timestamp":1368490067000},"page":"C673-C683","source":"Crossref","is-referenced-by-count":39,"title":["Expression, localization, and functional evaluation of  CFTR in bovine corneal endothelial cells"],"prefix":"10.1152","volume":"282","author":[{"given":"Xing Cai","family":"Sun","sequence":"first","affiliation":[{"name":"Indiana University School of Optometry, Bloomington, Indiana 47405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Bonanno","sequence":"additional","affiliation":[{"name":"Indiana University School of Optometry, Bloomington, Indiana 47405"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/0039-6257(93)90099-S"},{"key":"B2","first-page":"464","volume":"296","author":"Al-Nakkash L","year":"2001","journal-title":"J Pharmacol Exp Ther"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1021\/bi00246a001"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.3.C545"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0290"},{"key":"B6","first-page":"3058","volume":"33","author":"Bonanno JA","year":"1992","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B7","first-page":"2713","volume":"39","author":"Bonanno JA","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.113.5.743"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/S0302-4598(00)00096-9"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199912)181:3<489::AID-JCP13>3.0.CO;2-7"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1974.sp010676"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1007\/BF01869942"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.38.26946"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.2.C383"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.3.C441"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1974.sp010435"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1976.sp011645"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.275.5.L902"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.268.4.C886"},{"key":"B20","first-page":"488","volume":"41","author":"Jelamskii S","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.2.C449"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.280.3.C491"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(90)90037-U"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.278.2.C436"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)73367-7"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3109\/02713688208997692"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2001.280.4.L739"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.1.C108"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90313-5"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115358"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1042"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1972.sp009737"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1993.264.6.F1060"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.1.C98"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.12.5340"},{"key":"B36","first-page":"103","volume":"36","author":"Riley M","year":"1995","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B37","first-page":"1","volume":"37","author":"Riley M","year":"1996","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1480"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.5.C1464"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1995.269.6.G874"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(00)00075-6"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1999.79.1.S23"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.1.C16"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115696"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.5.C1648"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1076\/ceyr.22.1.1.6981"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1021\/bi00578a012"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1998.275.6.L1219"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.111.3.477"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.262.5.C1167"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050160"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(00)00089-2"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00384.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:11:48Z","timestamp":1718651508000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00384.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4,1]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4,1]]}},"alternative-id":["10.1152\/ajpcell.00384.2001"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00384.2001","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4,1]]}}}