{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T02:18:46Z","timestamp":1777688326979,"version":"3.51.4"},"reference-count":40,"publisher":"American Physiological Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:p> Interstitial cells of Cajal (ICC) generate slow waves in gastrointestinal (GI) muscles. Previous studies have suggested that slow wave generation and propagation depends on a voltage-dependent Ca<jats:sup>2+<\/jats:sup> entry mechanism with the signature of a T-type Ca<jats:sup>2+<\/jats:sup> conductance. We studied voltage-dependent inward currents in isolated ICC. ICC displayed two phases of inward current upon depolarization: a low voltage-activated inward current and a high voltage-activated current. The latter was of smaller current density and blocked by nicardipine. Ni<jats:sup>2+<\/jats:sup> (30 \u03bcM) or mibefradil (1 \u03bcM) blocked the low voltage-activated current. Replacement of extracellular Ca<jats:sup>2+<\/jats:sup> with Ba<jats:sup>2+<\/jats:sup> did not affect the current, suggesting that either charge carrier was equally permeable. Half-activation and half-inactivation occurred at \u221236 and \u221259 mV, respectively. Temperature sensitivity of the Ca<jats:sup>2+<\/jats:sup> current was also characterized. Increasing temperature (20\u201330\u00b0C) augmented peak current from \u22127 to \u221219 pA and decreased the activation time from 20.6 to 7.5 ms [temperature coefficient (Q<jats:sub>10<\/jats:sub>) = 3.0]. Molecular studies showed expression of Cacna1g (Cav3.1) and Cacna1h (Cav3.2) in ICC. The temperature dependence of slow waves in intact jejunal muscles of wild-type and Cacna1h<jats:sup> \u2212\/\u2212<\/jats:sup> mice was tested. Reducing temperature decreased the upstroke velocity significantly. Upstroke velocity was also reduced in muscles of Cacna1h<jats:sup> \u2212\/\u2212<\/jats:sup> mice, and Ni<jats:sup>2+<\/jats:sup> or reduced temperature had little effect on these muscles. Our data show that a T-type conductance is expressed and functional in ICC. With previous studies our data suggest that T-type current is required for entrainment of pacemaker activity within ICC and for active propagation of slow waves in ICC networks. <\/jats:p>","DOI":"10.1152\/ajpcell.00390.2013","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T05:21:26Z","timestamp":1391059286000},"page":"C705-C713","source":"Crossref","is-referenced-by-count":50,"title":["Expression and function of a T-type Ca<sup>2+<\/sup> conductance in interstitial cells of Cajal of the murine small intestine"],"prefix":"10.1152","volume":"306","author":[{"given":"Haifeng","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada, School of Medicine, Reno, Nevada; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Sik","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Don","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, University of Nevada, School of Medicine, Reno, Nevada; 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