{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:28:16Z","timestamp":1787563696644,"version":"build-2736575974"},"reference-count":51,"publisher":"American Physiological Society","issue":"5","funder":[{"name":"NEI","award":["EY009532"],"award-info":[{"award-number":["EY009532"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:p>\n                    The porcine lens response to a hyperosmotic stimulus involves an increase in the activity of an ion cotransporter sodium-potassium\/two-chloride cotransporter 1 (NKCC1). Recent studies with agonists and antagonists pointed to a mechanism that appears to depend on activation of transient receptor potential vanilloid 1 (TRPV1) ion channels. Here, we compare responses in lenses and cultured lens epithelium obtained from TRPV1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    and wild type (WT) mice. Hydrostatic pressure (HP) in lens surface cells was determined using a manometer-coupled microelectrode approach. The TRPV1 agonist capsaicin (100 nM) caused a transient HP increase in WT lenses that peaked after \u223c30 min and then returned toward baseline. Capsaicin did not cause a detectable change of HP in TRPV1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    lenses. The NKCC inhibitor bumetanide prevented the HP response to capsaicin in WT lenses. Potassium transport was examined by measuring Rb\n                    <jats:sup>+<\/jats:sup>\n                    uptake. Capsaicin increased Rb\n                    <jats:sup>+<\/jats:sup>\n                    uptake in cultured WT lens epithelial cells but not in TRPV1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    cells. Bumetanide, A889425, and the Akt inhibitor Akti prevented the Rb\n                    <jats:sup>+<\/jats:sup>\n                    uptake response to capsaicin. The bumetanide-sensitive (NKCC-dependent) component of Rb\n                    <jats:sup>+<\/jats:sup>\n                    uptake more than doubled in response to capsaicin. Capsaicin also elicited rapid (&lt;2 min) NKCC1 phosphorylation in WT but not TRPV1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    cells. HP recovery was shown to be absent in TRPV1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    lenses exposed to hyperosmotic solution. Bumetanide and Akti prevented HP recovery in WT lenses exposed to hyperosmotic solution. Taken together, responses to capsaicin and hyperosmotic solution point to a functional role for TRPV1 channels in mouse lens. Lack of NKCC1 phosphorylation and Rb\n                    <jats:sup>+<\/jats:sup>\n                    uptake responses in TRPV1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mouse epithelium reinforces the notion that a hyperosmotic challenge causes TRPV1-dependent NKCC1 activation. The results are consistent with a role for the TRPV1-activated signaling pathway leading to NKCC1 stimulation in lens osmotic homeostasis.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00391.2019","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T13:43:31Z","timestamp":1586958211000},"page":"C969-C980","source":"Crossref","is-referenced-by-count":25,"title":["TRPV1 activation stimulates NKCC1 and increases hydrostatic pressure in the mouse lens"],"prefix":"10.1152","volume":"318","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1142-4242","authenticated-orcid":false,"given":"Mohammad","family":"Shahidullah","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Arizona, Tucson, Arizona"},{"name":"Department of Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amritlal","family":"Mandal","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Arizona, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard T.","family":"Mathias","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Stony Brook University, Stony Brook, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4468-3029","authenticated-orcid":false,"given":"David","family":"Kri\u017eaj","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Vision Science, University of Utah School of Medicine, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Redmon","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Vision Science, University of Utah School of Medicine, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Delamere","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Arizona, Tucson, Arizona"},{"name":"Department of Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2005365"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604607103"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.03.040"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2010.0302"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1988.47"},{"key":"B6","first-page":"2199","volume":"46","author":"Bildin VN","year":"2005","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00483.2011"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10295"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"B10","first-page":"800","volume":"16","author":"Chee KN","year":"2010","journal-title":"Mol Vis"},{"key":"B11","first-page":"2574","volume":"41","author":"Crook RB","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20131478"},{"key":"B13","first-page":"2159","volume":"34","author":"Delamere NA","year":"1993","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B14","first-page":"2091","volume":"60","author":"Delamere NA","year":"2019","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2016.0054"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.05.005"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20071324"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.04.034"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1152\/physiologyonline.2001.16.3.118"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2016.09.002"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.12-10217"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201010538"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2015.09.018"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1007\/s002320010017"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00440.2002"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00392"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1959.197.6.1297"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.01.012"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2018.04.006"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-007-9019-y"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.2174\/157015906775202995"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2019.107707"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022246"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.68.040204.101406"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208108200"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.3390\/cells3030914"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.2741\/3651"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1172\/JCI70437"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.21074"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22858"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00252.2018"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.08.013"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00010.2012"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1038\/nn1614"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.03-0287"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1113\/JP274135"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00173.2011"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2017.08.009"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00016.2015"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-016-0353-7"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.08.061"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00391.2019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T12:20:56Z","timestamp":1718626856000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00391.2019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,1]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,1]]}},"alternative-id":["10.1152\/ajpcell.00391.2019"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00391.2019","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,1]]}}}