{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T06:56:21Z","timestamp":1774940181926,"version":"3.50.1"},"reference-count":44,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:p>Phospholamban (PLB) inhibits the sarcoplasmic reticulum (SR) Ca<jats:sup>2+<\/jats:sup>-ATPase (SERCA), and this inhibition is relieved by Ca<jats:sup>2+<\/jats:sup>calmodulin-dependent protein kinase II (CaM kinase II) phosphorylation. We previously reported significant differences in contractility, SR Ca<jats:sup>2+<\/jats:sup>release, and CaM kinase II activity in gastric fundus smooth muscles as a result of PLB phosphorylation by CaM kinase II. In this study, we used PLB-knockout (PLB-KO) mice to directly examine the effect of PLB absence on contractility, CaM kinase II activity, and intracellular Ca<jats:sup>2+<\/jats:sup>waves in gastric antrum smooth muscles. The frequencies and amplitudes of spontaneous phasic contractions were elevated in antrum smooth muscle strips from PLB-KO mice. Bethanecol increased the amplitudes of phasic contractions in antrum smooth muscles from both control and PLB-KO mice. Caffeine decreased and cyclopiazonic acid (CPA) increased the basal tone of antrum smooth muscle strips from PLB-KO mice, but the effects were less pronounced compared with control strips. The CaM kinase II inhibitor KN-93 was less effective at inhibiting caffeine-induced relaxation in antrum smooth muscle strips from PLB-KO mice. CaM kinase II autonomous activity was elevated, and not further increased by caffeine, in antrum smooth muscles from PLB-KO mice. Similarly, the intracellular Ca<jats:sup>2+<\/jats:sup>wave frequency was elevated, and not further increased by caffeine, in antrum smooth muscles from PLB-KO mice. These findings suggest that PLB is an important modulator of gastric antrum smooth muscle contractility by modulation of SR Ca<jats:sup>2+<\/jats:sup>release and CaM kinase II activity.<\/jats:p>","DOI":"10.1152\/ajpcell.00418.2007","type":"journal-article","created":{"date-parts":[[2007,11,29]],"date-time":"2007-11-29T01:34:47Z","timestamp":1196300087000},"page":"C432-C441","source":"Crossref","is-referenced-by-count":22,"title":["Phospholamban knockout increases CaM kinase II activity and intracellular Ca<sup>2+<\/sup>wave activity and alters contractile responses of murine gastric antrum"],"prefix":"10.1152","volume":"294","author":[{"given":"Minkyung","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grant W.","family":"Hennig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terence 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