{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T14:30:48Z","timestamp":1772029848542,"version":"3.50.1"},"reference-count":53,"publisher":"American Physiological Society","issue":"5","funder":[{"name":"the topic of Neurobehavioral in Zhengzhou University","award":["2016N07"],"award-info":[{"award-number":["2016N07"]}]},{"name":"the Science and Technology Program in Henan Province","award":["201702099"],"award-info":[{"award-number":["201702099"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2019,5,1]]},"abstract":"<jats:p> Accumulating evidence has highlighted the potential of microRNAs (miRs) as biomarkers in various human diseases. However, the roles of miRs in bacterial meningitis (BM), a severe infectious condition, still remain unclear. Thus, the present study aimed to investigate the effects of miR-135a on proliferation and apoptosis of astrocytes in BM. Neonatal rats were injected with Streptococcus pneumoniae to establish the BM model. The expression of miR-135a and hypoxia-inducible factor 1\u03b1 (HIF-1\u03b1) in the BM rat models were characterized, followed by determination of their interaction. Using gain- and loss-of-function approaches, the effects of miR-135a on proliferation, apoptosis, and expression of glial fibrillary acidic protein (GFAP), in addition to apoptosis-related factors in astrocytes were examined accordingly. The regulatory effect of HIF-1\u03b1 was also determined along with the overexpression or knockdown of HIF-1\u03b1. The results obtained indicated that miR-135a was poorly expressed, whereas HIF-1\u03b1 was highly expressed in the BM rat models. In addition, restored expression levels of miR-135a were determined to promote proliferation while inhibiting the apoptosis of astrocytes, along with downregulated Bax and Bad, as well as upregulated Bcl-2, Bcl-XL, and GFAP. As a target gene of miR-135a, HIF-1\u03b1 expression was determined to be diminished by miR-135a. The upregulation of HIF-1\u03b1 reversed the miR-135a-induced proliferation of astrocytes. Taken together, the key findings of the current study present evidence suggesting that miR-135a can downregulate HIF-1\u03b1 and play a contributory role in the development of astrocytes derived from BM, providing a novel theoretical perspective for BM treatment approaches. <\/jats:p>","DOI":"10.1152\/ajpcell.00440.2018","type":"journal-article","created":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T20:18:51Z","timestamp":1549484331000},"page":"C711-C721","source":"Crossref","is-referenced-by-count":15,"title":["MicroRNA-135a participates in the development of astrocytes derived from bacterial meningitis by downregulating HIF-1\u03b1"],"prefix":"10.1152","volume":"316","author":[{"given":"Yan","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China"},{"name":"Henan Provincial Key Laboratory of Child Brain Injury, Zhengzhou, 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