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In this study, we disrupted IFT81 expression in male germ cells starting from the spermatocyte stage. As a result, homozygous mutant males were completely infertile and displayed abnormal sperm parameters. In addition to oligozoospermia, spermatozoa presented dysmorphic and nonfunctional flagella. Histological examination of testes from homozygous mutant mice revealed abnormal spermiogenesis associated with sloughing of germ cells and the presence of numerous multinucleated giant germ cells (symblasts) in the lumen of seminiferous tubules and epididymis. Moreover, only few elongated spermatids and spermatozoa were seen in analyzed cross sections. Transmission electron microscopy showed a complete disorganization of the axoneme and para-axonemal structures such as the mitochondrial sheath, fibrous sheath, and outer dense fibers. In addition, numerous vesicles that contain unassembled microtubules were observed within developing spermatids. Acrosome structure analysis showed normal appearance, thus excluding a crucial role of IFT81 in acrosome biogenesis. These observations showed that IFT81 is an important member of the IFT process during spermatogenesis and that its absence is associated with abnormal flagellum formation leading to male infertility. The expression levels of several IFT components in testes, including IFT20, IFT25, IFT27, IFT57, IFT74, and IFT88, but not IFT140, were significantly reduced in homozygous mutant mice. Overall, our study demonstrates that IFT81 plays an essential role during spermatogenesis by modulating the assembly and elongation of the sperm flagella. <\/jats:p>","DOI":"10.1152\/ajpcell.00450.2019","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T19:20:20Z","timestamp":1585768820000},"page":"C1092-C1106","source":"Crossref","is-referenced-by-count":45,"title":["The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility"],"prefix":"10.1152","volume":"318","author":[{"given":"Wei","family":"Qu","sequence":"first","affiliation":[{"name":"School of Public Health, Wuhan University of Science and Technology, Wuhan, Hubei, China"},{"name":"Department of Physiology, Wayne State University, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Public Health, Wuhan University of Science and Technology, 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