{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:39:34Z","timestamp":1770914374879,"version":"3.50.1"},"reference-count":43,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:p>Relatively little is known about the contribution of Ca<jats:sup>2+<\/jats:sup>-dependent and -independent mechanisms in the contractility of neonatal gastrointestinal smooth muscle. We therefore studied Ca<jats:sup>2+<\/jats:sup>homeostasis and Ca<jats:sup>2+<\/jats:sup>sensitization mechanisms in 10-day-old and adult guinea pig gallbladder smooth muscle to elucidate developmental changes in these processes. Gallbladder contractility was evaluated by isometrical tension recordings from strips, intracellular Ca<jats:sup>2+<\/jats:sup>concentration was estimated by epifluorescence microscopy of fura-2-loaded isolated cells, and protein expression and phosphorylation were assessed by Western blot analysis. The neonatal gallbladder contracted significantly less to CCK than adult tissue, but this correlated with an increased Ca<jats:sup>2+<\/jats:sup>mobilization, suggesting immaturity of Ca<jats:sup>2+<\/jats:sup>sensitization mechanisms. The enhanced Ca<jats:sup>2+<\/jats:sup>release in the newborn gallbladder was the result of the increase in the size of the releasable Ca<jats:sup>2+<\/jats:sup>pool. Moreover, in neonatal smooth muscle cells, neither the plasma membrane Ca<jats:sup>2+<\/jats:sup>pump nor the Na<jats:sup>+<\/jats:sup>\/Ca<jats:sup>2+<\/jats:sup>exchanger collaborate in the extrusion of Ca<jats:sup>2+<\/jats:sup>. In contrast, in these cells, there is an increase in phospholamban phosphorylation, which could drive to an overactivity of the sarco(endo)plasmic reticulum Ca<jats:sup>2+<\/jats:sup>-ATPase pump. The reduced Ca<jats:sup>2+<\/jats:sup>sensitivity in neonatal tissues was demonstrated by the lack of effect to Y-27362, an inhibitor of Rho kinase (ROCK), and GF-109203X, an inhibitor of PKC, on agonist-induced contraction. In addition, the neonatal gallbladder showed lower levels of RhoA, ROCK, PKC, and two effectors [C-kinase-dependent inhibitor of 17 kDa (CPI-17) and myosin phosphatase targetting 1 (MYPT1)] as well as an absence of CPI-17 and MYPT1 phosphorylation in response to agonists. In conclusion, our results indicate that the main mechanisms involved in smooth muscle contractility are under developmental regulation.<\/jats:p>","DOI":"10.1152\/ajpcell.00452.2008","type":"journal-article","created":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T02:49:21Z","timestamp":1234406961000},"page":"C783-C791","source":"Crossref","is-referenced-by-count":15,"title":["Developmental changes in Ca<sup>2+<\/sup>homeostasis and contractility in gallbladder smooth muscle"],"prefix":"10.1152","volume":"296","author":[{"given":"Cristina","family":"Camello-Almaraz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Macias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro J.","family":"Gomez-Pinilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soledad","family":"Alcon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco E.","family":"Martin-Cano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akemishi","family":"Baba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro J.","family":"Camello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda J.","family":"Pozo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Akopov SE, Zhang L, Pearce WJ.Physiological variations in ovine cerebrovascular calcium sensitivity.Am J Physiol Heart Circ Physiol272: H2271\u2013H2281, 1997.","DOI":"10.1152\/ajpheart.1997.272.5.H2271"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Akopov SE, Zhang L, Pearce WJ.Regulation of Ca2+sensitization by PKC and rho proteins in ovine cerebral arteries: effects of artery size and age.Am J Physiol Heart Circ Physiol275: H930\u2013H939, 1998.","DOI":"10.1152\/ajpheart.1998.275.3.H930"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Arens Y, Chapados RA, Cox BE, Kamm KE, Rosenfeld CR.Differential development of umbilical and systemic arteries.II. 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