{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:46:38Z","timestamp":1760298398330},"reference-count":42,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p> We have characterized the native voltage-dependent K<jats:sup>+<\/jats:sup> (K<jats:sub>v<\/jats:sub>) current in rabbit urethral smooth muscle cells (RUSMC) and compared its pharmacological and biophysical properties with K<jats:sub>v<\/jats:sub>2.1 and K<jats:sub>v<\/jats:sub>2.2 channels cloned from the rabbit urethra and stably expressed in human embryonic kidney (HEK)-293 cells (HEK<jats:sub>Kv2.1<\/jats:sub> and HEK<jats:sub>Kv2.2<\/jats:sub>). RUSMC were perfused with Hanks\u2032 solution at 37\u00b0C and studied using the patch-clamp technique with K<jats:sup>+<\/jats:sup>-rich pipette solutions. Cells were bathed in 100 nM Penitrem A (Pen A) to block large-conductance Ca<jats:sup>2+<\/jats:sup>-activated K<jats:sup>+<\/jats:sup> (BK) currents and depolarized to +40 mV for 500 ms to evoke K<jats:sub>v<\/jats:sub> currents. These were unaffected by margatoxin, \u03ba-dendrotoxin, or \u03b1-dendrotoxin (100 nM, n = 3\u20135) but were blocked by stromatoxin-1 (ScTx, IC<jats:sub>50<\/jats:sub> \u223c130 nM), consistent with the idea that the currents were carried through K<jats:sub>v<\/jats:sub>2 channels. RNA was detected for K<jats:sub>v<\/jats:sub>2.1, K<jats:sub>v<\/jats:sub>2.2, and the silent subunit K<jats:sub>v<\/jats:sub>9.3 in urethral smooth muscle. Immunocytochemistry showed membrane staining for both K<jats:sub>v<\/jats:sub>2 subtypes and K<jats:sub>v<\/jats:sub>9.3 in isolated RUSMC. HEK<jats:sub>Kv2.1<\/jats:sub> and HEK<jats:sub>Kv2.2<\/jats:sub> currents were blocked in a concentration-dependent manner by ScTx, with estimated IC<jats:sub>50<\/jats:sub> values of \u223c150 nM (K<jats:sub>v<\/jats:sub>2.1, n = 5) and 70 nM (K<jats:sub>v<\/jats:sub>2.2, n = 6). The mean half-maximal voltage ( V<jats:sub>1\/2<\/jats:sub>) of inactivation of the USMC K<jats:sub>v<\/jats:sub> current was \u221256 \u00b1 3 mV ( n = 9). This was similar to the HEK<jats:sub>Kv2.1<\/jats:sub> current (\u221255 \u00b1 3 mV, n = 13) but significantly different from the HEK<jats:sub>Kv2.2<\/jats:sub> currents (\u221230 \u00b1 3 mV, n = 11). Action potentials (AP) evoked from RUSMC studied under current-clamp mode were unaffected by ScTx. However, when ScTx was applied in the presence of Pen A, the AP duration was significantly prolonged. Similarly, ScTx increased the amplitude of spontaneous contractions threefold, but only after Pen A application. These data suggest that K<jats:sub>v<\/jats:sub>2.1 channels contribute significantly to the K<jats:sub>v<\/jats:sub> current in RUSMC. <\/jats:p>","DOI":"10.1152\/ajpcell.00455.2010","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T05:32:27Z","timestamp":1312435947000},"page":"C1186-C1200","source":"Crossref","is-referenced-by-count":15,"title":["Contribution of K<sub>v<\/sub>2.1 channels to the delayed rectifier current in freshly dispersed smooth muscle cells from rabbit urethra"],"prefix":"10.1152","volume":"301","author":[{"given":"B.","family":"Kyle","sequence":"first","affiliation":[{"name":"The Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Bradley","sequence":"additional","affiliation":[{"name":"The Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Ohya","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. P.","family":"Sergeant","sequence":"additional","affiliation":[{"name":"The Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. G.","family":"McHale","sequence":"additional","affiliation":[{"name":"The Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. D.","family":"Thornbury","sequence":"additional","affiliation":[{"name":"The Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Hollywood","sequence":"additional","affiliation":[{"name":"The Smooth Muscle Research Centre, Dundalk Institute of Technology, Dundalk, Ireland; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.040014"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00086.2006"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.025171"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI333"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.097311"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00463.2003"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00036.2010"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/S1569-2590(00)08010-1"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.121bc.x"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1124\/mol.62.1.48"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.128454"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.10"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9659"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(00)00162-8"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0315a.x"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.279.2.C420"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.043695"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.153734"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1501"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1038\/nn1260"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4620-05.2006"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.165217"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1540\/jsmr.36.101"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122617999"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.22.6615"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01316-6"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(91)90017-T"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00211-6"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24371"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)02267-6"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.274.5.G901"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-2-00359.x"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15448.x"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701777"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1007\/PL00005071"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.039859"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00991.2008"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.01.004"},{"key":"B40","first-page":"543P S154","author":"Woolsey S","year":"2002","journal-title":"J Physiol"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.196618"},{"key":"B42","first-page":"337","volume":"6","author":"Zhu XR","year":"1999","journal-title":"Receptors Channels"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00455.2010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T16:56:33Z","timestamp":1718643393000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00455.2010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1152\/ajpcell.00455.2010"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00455.2010","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11]]}}}