{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:40:30Z","timestamp":1718653230885},"reference-count":36,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,8,1]]},"abstract":"<jats:p> Angiotensin (ANG) II receptors have been reported in the nonpigmented ciliary epithelium (NPE) of the eye. In cultured NPE, we found ANG II caused a dose-dependent rise of cytoplasmic sodium. The sodium increase was inhibited by the AT<jats:sub>1<\/jats:sub>-AT<jats:sub>2<\/jats:sub> receptor antagonist saralasin (IC<jats:sub>50<\/jats:sub> = 3.7 nM) and the AT<jats:sub>1<\/jats:sub> antagonist losartan (IC<jats:sub>50<\/jats:sub> = 0.6 nM) but not by the AT<jats:sub>2<\/jats:sub> antagonist PD-123319. ANG II also caused a dose-dependent increase in the rate of ouabain-sensitive<jats:sup>86<\/jats:sup>Rb uptake. The ANG II-induced cell sodium increase and<jats:sup>86<\/jats:sup>Rb uptake increase were reduced by dimethylamiloride (DMA; 10 \u03bcM). On the basis of this finding, we propose that Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchange is stimulated by ANG II. Simultaneously, ANG II appears to inhibit H<jats:sup>+<\/jats:sup>-ATPase-mediated proton export. Thus Ang II (10 nM) did not alter the baseline cytoplasmic pH (pH<jats:sub>i<\/jats:sub>) but reduced pH<jats:sub>i<\/jats:sub> in cells that were also exposed to 10 \u03bcM DMA. Consistent with the notion of H<jats:sup>+<\/jats:sup>-ATPase inhibition in ANG II-treated NPE, bafilomycin A<jats:sub>1<\/jats:sub> (100 nM) (BAF) and ANG II were both observed to suppress the pH<jats:sub>i<\/jats:sub> increase that occurs upon exposure to a mixture of epinephrine (1 \u03bcM) and acetylcholine (10 \u03bcM) and the pH<jats:sub>i<\/jats:sub> increase elicited by depolarization. In ATP hydrolysis measurements, H<jats:sup>+<\/jats:sup>-ATPase activity (bafilomycin A<jats:sub>1<\/jats:sub>-sensitive ATP hydrolysis) was reduced significantly in cells that had been pretreated 10 min with 10 nM ANG II. In summary, these studies suggest that ANG II causes H<jats:sup>+<\/jats:sup>-ATPase inhibition and an increase of cell sodium due to activation of Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchange. <\/jats:p>","DOI":"10.1152\/ajpcell.00459.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:09:41Z","timestamp":1368490181000},"page":"C552-C559","source":"Crossref","is-referenced-by-count":16,"title":["Influence of ANG II on cytoplasmic sodium in cultured  rabbit nonpigmented ciliary epithelium"],"prefix":"10.1152","volume":"283","author":[{"given":"Yining","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences and Department of Pharmacology and Toxicology, University of Louisville, School of Medicine, Louisville, Kentucky 40292"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas A.","family":"Delamere","sequence":"additional","affiliation":[{"name":"Department 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