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The ocular pulse induces pulsatile shear stress in Schlemm\u2019s canal (SC). We hypothesize that the ocular pulse modulates outflow facility by stimulating shear-induced nitric oxide (NO) production by SC cells. We confirmed that living mice exhibit an ocular pulse with a peak-to-peak (pk-pk) amplitude of 0.5\u2009mmHg under anesthesia. Using iPerfusion, we measured outflow facility (flow\/pressure) during alternating periods of steady or pulsatile IOP in both eyes of 16 cadaveric C57BL\/6J mice (13-14\u2009weeks). Eyes were retained in situ, with an applied mean pressure of 8\u2009mmHg and 1.0\u2009mmHg pk-pk pressure amplitude at 10\u2009Hz to mimic the murine heart rate. One eye of each cadaver was perfused with 100\u2009\u00b5M L-NAME to inhibit NO synthase, whereas the contralateral eye was perfused with vehicle. During the pulsatile period in the vehicle-treated eye, outflow facility increased by 16 [12, 20] % ( P &lt; 0.001) relative to the facility measured during the preceding and subsequent steady periods. This effect was partly inhibited by L-NAME, where pressure pulsations increased outflow facility by 8% [4, 12] ( P &lt; 0.001). Thus, the ocular pulse causes an immediate increase in outflow facility in mice, with roughly one-half of the facility increase attributable to NO production. These studies reveal a dynamic component to outflow function that responds instantly to the ocular pulse and may be important for outflow regulation and IOP homeostasis. <\/jats:p>","DOI":"10.1152\/ajpcell.00473.2020","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T19:50:37Z","timestamp":1610567437000},"page":"C652-C665","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["The ocular pulse decreases aqueous humor outflow resistance by stimulating nitric oxide production"],"prefix":"10.1152","volume":"320","author":[{"given":"Michael","family":"Madekurozwa","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Imperial College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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