{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:47:56Z","timestamp":1791978476655,"version":"4.4.0"},"reference-count":53,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:p> Absorption of dietary iodide, presumably in the small intestine, is the first step in iodide (I<jats:sup>\u2212<\/jats:sup>) utilization. From the bloodstream, I<jats:sup>\u2212<\/jats:sup> is actively taken up via the Na<jats:sup>+<\/jats:sup>\/I<jats:sup>\u2212<\/jats:sup> symporter (NIS) in the thyroid for thyroid hormone biosynthesis and in such other tissues as lactating breast, which supplies I<jats:sup>\u2212<\/jats:sup> to the newborn in the milk. The molecular basis for intestinal I<jats:sup>\u2212<\/jats:sup> absorption is unknown. We sought to determine whether I<jats:sup>\u2212<\/jats:sup> is actively accumulated by enterocytes and, if so, whether this process is mediated by NIS and regulated by I<jats:sup>\u2212<\/jats:sup> itself. NIS expression was localized exclusively at the apical surface of rat and mouse enterocytes. In vivo intestine-to-blood transport of pertechnetate, a NIS substrate, was sensitive to the NIS inhibitor perchlorate. Brush border membrane vesicles accumulated I<jats:sup>\u2212<\/jats:sup> in a sodium-dependent, perchlorate-sensitive manner with kinetic parameters similar to those of thyroid cells. NIS was expressed in intestinal epithelial cell line 6, and I<jats:sup>\u2212<\/jats:sup> uptake in these cells was also kinetically similar to that in thyrocytes. I<jats:sup>\u2212<\/jats:sup> downregulated NIS protein expression and its own NIS-mediated transport both in vitro and in vivo. We conclude that NIS is functionally expressed on the apical surface of enterocytes, where it mediates active I<jats:sup>\u2212<\/jats:sup> accumulation. Therefore, NIS is a significant and possibly central component of the I<jats:sup>\u2212<\/jats:sup> absorption system in the small intestine, a system of key importance for thyroid hormone biosynthesis and thus systemic intermediary metabolism. <\/jats:p>","DOI":"10.1152\/ajpcell.00509.2008","type":"journal-article","created":{"date-parts":[[2008,12,4]],"date-time":"2008-12-04T02:49:30Z","timestamp":1228358970000},"page":"C654-C662","source":"Crossref","is-referenced-by-count":140,"title":["The Na<sup>+<\/sup>\/I<sup>\u2212<\/sup> symporter mediates active iodide uptake in the intestine"],"prefix":"10.1152","volume":"296","author":[{"given":"Juan Pablo","family":"Nicola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Basquin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Portulano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Reyna-Neyra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Paroder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Carrasco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R3","doi-asserted-by":"crossref","unstructured":"Acland JD, Illman O. 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