{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:31:34Z","timestamp":1787589094336,"version":"build-2736575974"},"reference-count":58,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:p>Gelsolin is a highly conserved intracellular actin-binding protein with an extracellular isoform, plasma gelsolin (pGSN). Blood concentrations of pGSN decrease in response to diverse tissue injuries. Depletion of pGSN to critical levels precedes and often predicts complications of injuries such as lung permeability changes and death. Administration of recombinant pGSN ameliorates such complications and reduces mortality in animal models. One proposed mechanism for pGSN's protective effects is that it inhibits inflammatory mediators generated during primary injuries, since pGSN binds bioactive mediators, including lysophospatidic acid (LPA) and endotoxin in vitro. However, no direct evidence in support of this hypothesis has been available. Here we show that recombinant pGSN modestly inhibited LPA-induced P-selectin upregulation by human platelets in the presence of albumin ( P &lt; 0.0001). However, physiologically relevant pGSN concentrations inhibit platelet-activating factor (PAF)-mediated P-selectin expression by up to 77% ( P &lt; 0.0001). pGSN also markedly inhibited PAF-induced superoxide anion (O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>) production of human peripheral neutrophils (PMN) in a concentration-dependent manner ( P &lt; 0.0001). A phospholipid-binding peptide derived from pGSN (QRLFQVKGRR) also inhibited PAF-mediated O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup>generation ( P = 0.024). Therefore, pGSN interferes with PAF- and LPA-induced cellular activation in vitro, suggesting a mechanism for the protective role of pGSN in vivo.<\/jats:p>","DOI":"10.1152\/ajpcell.00510.2006","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T01:40:12Z","timestamp":1164850812000},"page":"C1323-C1330","source":"Crossref","is-referenced-by-count":80,"title":["Modifications of cellular responses to lysophosphatidic acid and platelet-activating factor by plasma gelsolin"],"prefix":"10.1152","volume":"292","author":[{"given":"Teresia M.","family":"Osborn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claes","family":"Dahlgren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Hartwig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Stossel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Barkalow K, Witke W, Kwiatkowski D, Hartwig J.Coordinated regulation of platelet actin filament barbed ends by gelsolin and capping protein.J Cell Biol134: 389\u2013399, 1996.","DOI":"10.1083\/jcb.134.2.389"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Boyum A, Lovhaug D, Tresland L, Nordlie EM.Separation of leucocytes: improved cell purity by fine adjustments of gradient medium density and osmolality.Scand J Immunol34: 697\u2013712, 1991.","DOI":"10.1111\/j.1365-3083.1991.tb01594.x"},{"key":"R3","doi-asserted-by":"crossref","unstructured":"Bucki R, Georges PC, Espinassous Q, Funaki M, Pastore JJ, Chaby R, Janmey PA.Inactivation of endotoxin by human plasma gelsolin.Biochemistry44: 9590\u20139597, 2005.","DOI":"10.1021\/bi0503504"},{"key":"R4","doi-asserted-by":"crossref","unstructured":"Chauhan VP, Ray I, Chauhan A, Wisniewski HM.Binding of gelsolin, a secretory protein, to amyloid beta-protein.Biochem Biophys Res Commun258: 241\u2013246, 1999.","DOI":"10.1006\/bbrc.1999.0623"},{"key":"R5","doi-asserted-by":"crossref","unstructured":"Christofidou-Solomidou M, Scherpereel A, Solomides CC, Christie JD, Stossel TP, Goelz S, DiNubile MJ.Recombinant plasma gelsolin diminishes the acute inflammatory response to hyperoxia in mice.J Investig Med50: 54\u201360, 2002.","DOI":"10.2310\/6650.2002.33518"},{"key":"R6","doi-asserted-by":"crossref","unstructured":"Christofidou-Solomidou M, Scherpereel A, Solomides CC, Muzykantov VR, Machtay M, Albelda SM, DiNubile MJ.Changes in plasma gelsolin levels during acute oxidant lung injury in mice.Lung180: 91\u2013104, 2002.","DOI":"10.1007\/s004080000084"},{"key":"R7","doi-asserted-by":"crossref","unstructured":"Clay KL, Johnson C, Henson P.Binding of platelet activating factor to albumin.Biochim Biophys Acta1046: 309\u2013314, 1990.","DOI":"10.1016\/0005-2760(90)90246-T"},{"key":"R8","doi-asserted-by":"crossref","unstructured":"Cooke NE, Haddad JG.Vitamin D binding protein (Gc-globulin).Endocr Rev10: 294\u2013307, 1989.","DOI":"10.1210\/edrv-10-3-294"},{"key":"R9","doi-asserted-by":"crossref","unstructured":"Cunningham CC, Vegners R, Bucki R, Funaki M, Korde N, Hartwig JH, Stossel TP, Janmey PA.Cell permeant polyphosphoinositide-binding peptides that block cell motility and actin assembly.J Biol Chem276: 43390\u201343399, 2001.","DOI":"10.1074\/jbc.M105289200"},{"key":"R10","doi-asserted-by":"crossref","unstructured":"Dahl B, Schiodt FV, Ott P, Gvozdenovic R, Yin HL, Lee WM.Plasma gelsolin is reduced in trauma patients.Shock12: 102\u2013104, 1999.","DOI":"10.1097\/00024382-199908000-00002"},{"key":"R11","doi-asserted-by":"crossref","unstructured":"Dahlgren C, Karlsson A.Respiratory burst in human neutrophils.J Immunol Methods232: 3\u201314, 1999.","DOI":"10.1016\/S0022-1759(99)00146-5"},{"key":"R12","doi-asserted-by":"crossref","unstructured":"Dhainaut JF, Tenaillon A, Hemmer M, Damas P, Le Tulzo Y, Radermacher P, Schaller MD, Sollet JP, Wolff M, Holzapfel L, Zeni F, Vedrinne JM, de Vathaire F, Gourlay ML, Guinot P, Mira JP.Confirmatory platelet-activating factor receptor antagonist trial in patients with severe gram-negative bacterial sepsis: a phase III, randomized, double-blind, placebo-controlled, multicenter trial. BN 52021 Sepsis Investigator Group.Crit Care Med26: 1963\u20131971, 1998.","DOI":"10.1097\/00003246-199812000-00021"},{"key":"R13","doi-asserted-by":"crossref","unstructured":"DiNubile MJ, Stossel TP, Ljunghusen OC, Ferrara JL, Antin JH.Prognostic implications of declining plasma gelsolin levels after allogeneic stem cell transplantation.Blood100: 4367\u20134371, 2002.","DOI":"10.1182\/blood-2002-06-1672"},{"key":"R14","doi-asserted-by":"crossref","unstructured":"Dueland S, Nenseter MS, Drevon CA.Uptake and degradation of filamentous actin and vitamin-D binding protein in the rat.Biochem J274: 237\u2013241, 1991.","DOI":"10.1042\/bj2740237"},{"key":"R15","doi-asserted-by":"crossref","unstructured":"Erukhimov JA, Tang ZL, Johnson BA, Donahoe MP, Razzack JA, Gibson KF, Lee WM, Wasserloos KJ, Watkins SA, Pitt BR.Actin-containing sera from patients with adult respiratory distress syndrome are toxic to sheep pulmonary endothelial cell.Am J Respir Crit Care Med162: 288\u2013294, 2000.","DOI":"10.1164\/ajrccm.162.1.9806088"},{"key":"R16","doi-asserted-by":"crossref","unstructured":"Falet H, Pain S, Rendu F.Tyrosine unphosphorylated platelet SHP-1 is a substrate for calpain.Biochem Biophys Res Commun252: 51\u201355, 1998.","DOI":"10.1006\/bbrc.1998.9593"},{"key":"R17","doi-asserted-by":"crossref","unstructured":"Feng L, Mejillano M, Yin HL, Chen J, Prestwich GD.Full-contact domain labeling: identification of a novel phosphoinositide binding site on gelsolin that requires the complete protein.Biochemistry40: 904\u2013913, 2001.","DOI":"10.1021\/bi000996q"},{"key":"R18","doi-asserted-by":"crossref","unstructured":"Fischer DJ, Nusser N, Virag T, Yokoyama K, Wang D, Baker DL, Bautista D, Parrill AL, Tigyi G.Short-chain phosphatidates are subtype-selective antagonists of lysophosphatidic acid receptors.Mol Pharmacol60: 776\u2013784, 2001.","DOI":"10.1016\/S0026-895X(24)12305-X"},{"key":"R19","doi-asserted-by":"crossref","unstructured":"Fisher CJ Jr, Agosti JM, Opal SM, Lowry SF, Balk RA, Sadoff JC, Abraham E, Schein RM, Benjamin E.Treatment of septic shock with the tumor necrosis factor receptor:Fc fusion protein. The Soluble TNF Receptor Sepsis Study Group.N Engl J Med334: 1697\u20131702, 1996.","DOI":"10.1056\/NEJM199606273342603"},{"key":"R20","doi-asserted-by":"crossref","unstructured":"Goetzl EJ, Lee H, Azuma T, Stossel TP, Turck CW, Karliner JS.Gelsolin binding and cellular presentation of lysophosphatidic acid.J Biol Chem275: 14573\u201314578, 2000.","DOI":"10.1074\/jbc.275.19.14573"},{"key":"R21","doi-asserted-by":"crossref","unstructured":"Graler MH, Goetzl EJ.Lysophospholipids and their G protein-coupled receptors in inflammation and immunity.Biochim Biophys Acta1582: 168\u2013174, 2002.","DOI":"10.1016\/S1388-1981(02)00152-X"},{"key":"R22","doi-asserted-by":"crossref","unstructured":"Haddad JG, Harper KD, Guoth M, Pietra GG, Sanger JW.Angiopathic consequences of saturating the plasma scavenger system for actin.Proc Natl Acad Sci USA87: 1381\u20131385, 1990.","DOI":"10.1073\/pnas.87.4.1381"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Hama K, Bandoh K, Kakehi Y, Aoki J, Arai H.Lysophosphatidic acid (LPA) receptors are activated differentially by biological fluids: possible role of LPA-binding proteins in activation of LPA receptors.FEBS Lett523: 187\u2013192, 2002.","DOI":"10.1016\/S0014-5793(02)02976-9"},{"key":"R24","doi-asserted-by":"crossref","unstructured":"Haseruck N, Erl W, Pandey D, Tigyi G, Ohlmann P, Ravanat C, Gachet C, Siess W.The plaque lipid lysophosphatidic acid stimulates platelet activation and platelet-monocyte aggregate formation in whole blood: involvement of P2Y1 and P2Y12 receptors.Blood103: 2585\u20132592, 2004.","DOI":"10.1182\/blood-2003-04-1127"},{"key":"R25","doi-asserted-by":"crossref","unstructured":"Huang S, Rhoads SL, DiNubile MJ.Temporal association between serum gelsolin levels and clinical events in a patient with severe falciparum malaria.Clin Infect Dis24: 951\u2013954, 1997.","DOI":"10.1093\/clinids\/24.5.951"},{"key":"R26","doi-asserted-by":"crossref","unstructured":"Izumi T, Shimizu T.Platelet-activating factor receptor: gene expression and signal transduction.Biochim Biophys Acta1259: 317\u2013333, 1995.","DOI":"10.1016\/0005-2760(95)00171-9"},{"key":"R27","doi-asserted-by":"crossref","unstructured":"Janmey PA, Lind SE, Yin HL, Stossel TP.Effects of semi-dilute actin solutions on the mobility of fibrin protofibrils during clot formation.Biochim Biophys Acta841: 151\u2013158, 1985.","DOI":"10.1016\/0304-4165(85)90016-9"},{"key":"R28","doi-asserted-by":"crossref","unstructured":"Janmey PA, Stossel TP.Modulation of gelsolin function by phosphatidylinositol 4,5-bisphosphate.Nature325: 362\u2013364, 1987.","DOI":"10.1038\/325362a0"},{"key":"R29","doi-asserted-by":"crossref","unstructured":"Kouyama T, Mihashi K.Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Local structural change of actin protomer both on polymerization and on binding of heavy meromyosin.Eur J Biochem114: 33\u201338, 1981.","DOI":"10.1111\/j.1432-1033.1981.tb06167.x"},{"key":"R30","doi-asserted-by":"crossref","unstructured":"Kwiatkowski DJ, Stossel TP, Orkin SH, Mole JE, Colten HR, Yin HL.Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.Nature323: 455\u2013458, 1986.","DOI":"10.1038\/323455a0"},{"key":"R31","doi-asserted-by":"crossref","unstructured":"Lee PS, Drager LR, Stossel TP, Moore FD, Rogers SO.Relationship of plasma gelsolin levels to outcomes in critically ill surgical patients.Ann Surg243: 399\u2013403, 2006.","DOI":"10.1097\/01.sla.0000201798.77133.55"},{"key":"R32","doi-asserted-by":"crossref","unstructured":"Lee PS, Waxman AB, Cotich BA, Chung SW, Perrella MA, Stossel TP.Plasma gelsolin is a marker and therapeutic agent in animal sepsis.Crit Care Med. First published Jan. 3, 2007; 10.1097\/01.CCM.0000253815.26311.24.","DOI":"10.1097\/01.CCM.0000253815.26311.24"},{"key":"R33","doi-asserted-by":"crossref","unstructured":"Lee WM, Galbraith RM.The extracellular actin-scavenger system and actin toxicity.N Engl J Med326: 1335\u20131341, 1992.","DOI":"10.1056\/NEJM199205143262006"},{"key":"R34","doi-asserted-by":"crossref","unstructured":"Lind SE, Smith DB, Janmey PA, Stossel TP.Depression of gelsolin levels and detection of gelsolin-actin complexes in plasma of patients with acute lung injury.Am Rev Respir Dis138: 429\u2013434, 1988.","DOI":"10.1164\/ajrccm\/138.2.429"},{"key":"R35","doi-asserted-by":"crossref","unstructured":"Lind SE, Smith DB, Janmey PA, Stossel TP.Role of plasma gelsolin and the vitamin D-binding protein in clearing actin from the circulation.J Clin Invest78: 736\u2013742, 1986.","DOI":"10.1172\/JCI112634"},{"key":"R36","doi-asserted-by":"crossref","unstructured":"Lopez Diez F, Nieto ML, Fernandez-Gallardo S, Gijon MA, Sanchez Crespo M.Occupancy of platelet receptors for platelet-activating factor in patients with septicemia.J Clin Invest83: 1733\u20131740, 1989.","DOI":"10.1172\/JCI114074"},{"key":"R37","doi-asserted-by":"crossref","unstructured":"Ludwig JC, Hoppens CL, McManus LM, Mott GE, Pinckard RN.Modulation of platelet-activating factor (PAF) synthesis and release from human polymorphonuclear leukocytes (PMN): role of extracellular albumin.Arch Biochem Biophys241: 337\u2013347, 1985.","DOI":"10.1016\/0003-9861(85)90555-7"},{"key":"R38","doi-asserted-by":"crossref","unstructured":"Ludwig JC, McManus LM, Clark PO, Hanahan DJ, Pinckard RN.Modulation of platelet-activating factor (PAF) synthesis and release from human polymorphonuclear leukocytes (PMN): role of extracellular Ca2+.Arch Biochem Biophys232: 102\u2013110, 1984.","DOI":"10.1016\/0003-9861(84)90525-3"},{"key":"R39","doi-asserted-by":"crossref","unstructured":"Mariano F, Guida G, Donati D, Tetta C, Cavalli PL, Verzetti G, Piccoli G, Camussi G.Production of platelet-activating factor in patients with sepsis-associated acute renal failure.Nephrol Dial Transplant14: 1150\u20131157, 1999.","DOI":"10.1093\/ndt\/14.5.1150"},{"key":"R40","doi-asserted-by":"crossref","unstructured":"Meerschaert K, De Corte V, De Ville Y, Vandekerckhove J, Gettemans J.Gelsolin and functionally similar actin-binding proteins are regulated by lysophosphatidic acid.EMBO J17: 5923\u20135932, 1998.","DOI":"10.1093\/emboj\/17.20.5923"},{"key":"R41","doi-asserted-by":"crossref","unstructured":"Mere J, Chahinian A, Maciver SK, Fattoum A, Bettache N, Benyamin Y, Roustan C.Gelsolin binds to polyphosphoinositide-free lipid vesicles and simultaneously to actin microfilaments.Biochem J386: 47\u201356, 2005.","DOI":"10.1042\/BJ20041054"},{"key":"R42","doi-asserted-by":"crossref","unstructured":"Mounzer KC, Moncure M, Smith YR, DiNubile MJ.Relationship of admission gelsolin levels to clinical outcomes in patients after major trauma.Am J Resp Dis Crit Care Med160: 1673\u20131681, 1999.","DOI":"10.1164\/ajrccm.160.5.9807137"},{"key":"R43","doi-asserted-by":"crossref","unstructured":"Nieto ML, Velasco S, Sanchez Crespo M.Biosynthesis of platelet-activating factor in human polymorphonuclear leukocytes. Involvement of the cholinephosphotransferase pathway in response to the phorbol esters.J Biol Chem263: 2217\u20132222, 1988.","DOI":"10.1016\/S0021-9258(18)69193-8"},{"key":"R44","doi-asserted-by":"crossref","unstructured":"Opal S, Laterre PF, Abraham E, Francois B, Wittebole X, Lowry S, Dhainaut JF, Warren B, Dugernier T, Lopez A, Sanchez M, Demeyer I, Jauregui L, Lorente JA, McGee W, Reinhart K, Kljucar S, Souza S, Pribble J, Controlled Mortality Trial of Platelet-Activating Factor Acetylhydrolase in Severe Sepsis (COMPASS) Investigators.Recombinant human platelet-activating factor acetylhydrolase for treatment of severe sepsis: results of a phase III, multicenter, randomized, double-blind, placebo-controlled, clinical trial.Crit Care Med32: 332\u2013341, 2004.","DOI":"10.1097\/01.CCM.0000108867.87890.6D"},{"key":"R45","doi-asserted-by":"crossref","unstructured":"Rothenbach PA, Dahl B, Schwartz JJ, O'Keefe GE, Yamamoto M, Lee WM, Horton JW, Yin HL, Turnage RH.Recombinant plasma gelsolin infusion attenuates burn-induced pulmonary microvascular dysfunction.J Appl Physiol96: 25\u201331, 2004.","DOI":"10.1152\/japplphysiol.01074.2002"},{"key":"R46","doi-asserted-by":"crossref","unstructured":"Schuster DP, Metzler M, Opal S, Lowry S, Balk R, Abraham E, Levy H, Slotman G, Coyne E, Souza S, Pribble J, Pafase ARDS Prevention Study Group.Recombinant platelet-activating factor acetylhydrolase to prevent acute respiratory distress syndrome and mortality in severe sepsis: Phase IIb, multicenter, randomized, placebo-controlled, clinical trial.Crit Care Med31: 1612\u20131619, 2003.","DOI":"10.1097\/01.CCM.0000063267.79824.DB"},{"key":"R47","doi-asserted-by":"crossref","unstructured":"Siess W, Tigyi G.Thrombogenic and atherogenic activities of lysophosphatidic acid.J Cell Biochem92: 1086\u20131094, 2004.","DOI":"10.1002\/jcb.20108"},{"key":"R48","doi-asserted-by":"crossref","unstructured":"Snyder F.Platelet-activating factor and related acetylated lipids as potent biologically active cellular mediators.Am J Physiol Cell Physiol259: C697\u2013C708, 1990.","DOI":"10.1152\/ajpcell.1990.259.5.C697"},{"key":"R49","doi-asserted-by":"crossref","unstructured":"Stafforini DM, McIntyre TM, Zimmerman GA, Prescott SM.Platelet-activating factor, a pleiotrophic mediator of physiological and pathological processes.Crit Rev Clin Lab Sci40: 643\u2013672, 2003.","DOI":"10.1080\/714037693"},{"key":"R50","doi-asserted-by":"crossref","unstructured":"Suhler E, Lin W, Yin HL, Lee WM.Decreased plasma gelsolin concentrations in acute liver failure, myocardial infarction, septic shock, and myonecrosis.Crit Care Med25: 594\u2013598, 1997.","DOI":"10.1097\/00003246-199704000-00007"},{"key":"R51","doi-asserted-by":"crossref","unstructured":"Suputtamongkol Y, Intaranongpai S, Smith MD, Angus B, Chaowagul W, Permpikul C, Simpson JA, Leelarasamee A, Curtis L, White NJ.A double-blind placebo-controlled study of an infusion of lexipafant (Platelet-activating factor receptor antagonist) in patients with severe sepsis.Antimicrob Agents Chemother44: 693\u2013696, 2000.","DOI":"10.1128\/AAC.44.3.693-696.2000"},{"key":"R52","doi-asserted-by":"crossref","unstructured":"Thumser AE, Voysey JE, Wilton DC.The binding of lysophospholipids to rat liver fatty acid-binding protein and albumin.Biochem J301: 801\u2013806, 1994.","DOI":"10.1042\/bj3010801"},{"key":"R53","doi-asserted-by":"crossref","unstructured":"Tigyi G, Miledi R.Lysophosphatidates bound to serum albumin activate membrane currents in Xenopus oocytes and neurite retraction in PC12 pheochromocytoma cells.J Biol Chem267: 21360\u201321367, 1992.","DOI":"10.1016\/S0021-9258(19)36618-9"},{"key":"R54","doi-asserted-by":"crossref","unstructured":"Tigyi G, Parrill AL.Molecular mechanisms of lysophosphatidic acid action.Prog Lipid Res42: 498\u2013526, 2003.","DOI":"10.1016\/S0163-7827(03)00035-3"},{"key":"R55","unstructured":"Vartanian AA.Gelsolin and plasminogen activator inhibitor-1 are Ap3A-binding proteins.Ital J Biochem52: 9\u201316, 2003."},{"key":"R56","doi-asserted-by":"crossref","unstructured":"Wen D, Corina K, Chow EP, Miller S, Janmey PA, Pepinsky RB.The plasma and cytoplasmic forms of human gelsolin differ in disulfide structure.Biochemistry35: 9700\u20139709, 1996.","DOI":"10.1021\/bi960920n"},{"key":"R57","unstructured":"Zeng S, Yi FX, Guo ZG.Platelet activating factor-induced P-selectin expression in platelets and its related signal transduction.Zhongguo Yao Li Xue Bao20: 948\u2013950, 1999."},{"key":"R58","doi-asserted-by":"crossref","unstructured":"Zimmerman GA, McIntyre TM, Prescott SM, Stafforini DM.The platelet-activating factor signaling system and its regulators in syndromes of inflammation and thrombosis.Crit Care Med30: S294\u2013S301, 2002.","DOI":"10.1097\/00003246-200205001-00020"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00510.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T06:00:16Z","timestamp":1736661616000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00510.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":58,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1152\/ajpcell.00510.2006"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00510.2006","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]}}}