{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T02:31:33Z","timestamp":1785292293468,"version":"3.55.0"},"reference-count":32,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p> The effect of ANG II on intracellular pH (pH<jats:sub>i<\/jats:sub>) recovery rate and AT<jats:sub>1<\/jats:sub> receptor translocation was investigated in transfected MDCK cells. The pH<jats:sub>i<\/jats:sub> recovery rate was evaluated by fluorescence microscopy using the fluorescent probe BCECF-AM. The human angiotensin II receptor isoform 1 (hAT<jats:sub>1<\/jats:sub>) translocation was analyzed by immunofluorescence and confocal microscope. Our data show that transfected cells in control situation have a pH<jats:sub>i<\/jats:sub> recovery rate of 0.219 \u00b1 0.017 pH U\/min ( n = 11). This value was similar to nontransfected cells [0.211 \u00b1 0.009 pH U\/min ( n = 12)]. Both values were significantly increased with ANG II (10<jats:sup>\u22129<\/jats:sup> M) but not with ANG II (10<jats:sup>\u22126<\/jats:sup> M). Losartan (10<jats:sup>\u22127<\/jats:sup> M) and dimethyl-BAPTA-AM (10<jats:sup>\u22127<\/jats:sup> M) decreased significantly the stimulatory effect of ANG II (10<jats:sup>\u22129<\/jats:sup> M) and induced an increase in Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> exchanger 1 (NHE-1) activity with ANG II (10<jats:sup>\u22126<\/jats:sup> M). Immunofluorescence studies indicated that in control situation, the hAT<jats:sub>1<\/jats:sub> receptor was predominantly expressed in cytosol. However, it was translocated to plasma membrane with ANG II (10<jats:sup>\u22129<\/jats:sup> M) and internalized with ANG II (10<jats:sup>\u22126<\/jats:sup> M). Losartan (10<jats:sup>\u22127<\/jats:sup> M) induced hAT<jats:sub>1<\/jats:sub> translocation to plasma membrane in all studied groups. Dimethyl-BAPTA-AM (10<jats:sup>\u22127<\/jats:sup> M) did not change the effect of ANG II (10<jats:sup>\u22129<\/jats:sup> M) on the hAT<jats:sub>1<\/jats:sub> receptor distribution but induced its accumulation at plasma membrane in cells treated with ANG II (10<jats:sup>\u22126<\/jats:sup> M). With ionomycin (10<jats:sup>\u22126<\/jats:sup> M), the receptor was accumulated in cytosol. The results indicate that, in MDCK cells, the effect of ANG II on NHE-1 activity is associated with ligand binding to AT<jats:sub>1<\/jats:sub> receptor and intracellular signaling events related to AT<jats:sub>1<\/jats:sub> translocation. <\/jats:p>","DOI":"10.1152\/ajpcell.00512.2007","type":"journal-article","created":{"date-parts":[[2008,4,24]],"date-time":"2008-04-24T01:26:53Z","timestamp":1209000413000},"page":"C138-C145","source":"Crossref","is-referenced-by-count":8,"title":["The effect of angiotensin II on intracellular pH is mediated by AT<sub>1<\/sub> receptor translocation"],"prefix":"10.1152","volume":"295","author":[{"given":"Karina","family":"Thieme","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e9bora Mai N.","family":"Eguti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarida","family":"Mello-Aires","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Oliveira-Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Aharonovitz O, Zaun HC, Balla T, York JD, Orlowski J, Grinstein S. 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