{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T01:37:39Z","timestamp":1770341859983,"version":"3.49.0"},"reference-count":62,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p>Partial urinary bladder outlet obstruction (PBOO) in men, secondary to benign prostatic hyperplasia, induces detrusor smooth muscle (DSM) hypertrophy. However, despite DSM hypertrophy, some bladders become severely dysfunctional (decompensated). Using a rabbit model of PBOO, we found that although DSM from sham-operated bladders expressed nearly 100% of both the smooth muscle myosin heavy chain isoform SM-B and essential light chain isoform LC<jats:sub>17a<\/jats:sub>, DSM from severely dysfunctional bladders expressed as much as 75% SM-A and 40% LC<jats:sub>17b<\/jats:sub>(both associated with decreased maximum velocity of shortening). DSM from dysfunctional bladder also exhibited tonic-type contractions, characterized by slow force generation and high force maintenance. Immunofluorescence microscopy showed that decreased SM-B expression in dysfunctional bladders was not due to generation of a new cell population lacking SM-B. Metabolic cage monitoring revealed decreased void volume and increased voiding frequency correlated with overexpression of SM-A and LC<jats:sub>17b<\/jats:sub>. Myosin isoform expression and bladder function returned toward normal upon removal of the obstruction, indicating that the levels of expression of these isoforms are markers of the PBOO-induced dysfunctional bladders.<\/jats:p>","DOI":"10.1152\/ajpcell.00513.2002","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:17:21Z","timestamp":1368490641000},"page":"C1397-C1410","source":"Crossref","is-referenced-by-count":72,"title":["Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction"],"prefix":"10.1152","volume":"285","author":[{"given":"Michael E.","family":"DiSanto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimund","family":"Stein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A.","family":"Hypolite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongmu","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Zderic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Wein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Chacko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"REF1","doi-asserted-by":"crossref","unstructured":"Adelstein RSand Eisenberg E.Regulation and kinetics of the actin-myosin-ATP interaction.Annu Rev Biochem49: 921-956, 1980.","DOI":"10.1146\/annurev.bi.49.070180.004421"},{"key":"REF2","unstructured":"Alberts B, Bray D, Lewis J, Raff M, Roberts K, and Watson J.Molecular Biology of the Cell. New York: Garland, 1983, p. 412."},{"key":"REF3","doi-asserted-by":"crossref","unstructured":"Babij P.Tissue-specific and developmentally regulated alternative splicing of a visceral isoform of smooth muscle myosin heavy chain.Nucleic Acids Res21: 1467-1471, 1993.","DOI":"10.1093\/nar\/21.6.1467"},{"key":"REF4","unstructured":"Babij P, Kelly C, and Periasamy M.Characterization of a mammalian smooth muscle myosin heavy-chain gene: complete nucleotide and protein coding sequence and analysis of the 5\u2032 end of the gene.Proc Natl Acad Sci USA88: 10676-10680, 1991."},{"key":"REF5","doi-asserted-by":"crossref","unstructured":"Babij Pand Periasamy M.Myosin heavy chain isoform diversity in smooth muscle is produced by differential RNA processing.J Mol Biol210: 673-679, 1989.","DOI":"10.1016\/0022-2836(89)90142-3"},{"key":"REF6","doi-asserted-by":"crossref","unstructured":"Babu GJ, Loukianov E, Loukianova T, Pyne GJ, Huke S, Osol G, Low RB, Paul RJ, and Periasamy M.Loss of SM-B myosin affects muscle shortening velocity and maximal force development.Nat Cell Biol3: 1025-1029, 2001.","DOI":"10.1038\/ncb1101-1025"},{"key":"REF7","doi-asserted-by":"crossref","unstructured":"Barany M.ATPase activity of myosin correlated with speed of muscle shortening.J Gen Physiol50,Suppl: 197-218, 1967.","DOI":"10.1085\/jgp.50.6.197"},{"key":"REF8","doi-asserted-by":"crossref","unstructured":"Bruskewitz RC, Reda DJ, Wasson JH, Barrett L, and Phelan M.Testing to predict outcome after transurethral resection of the prostate.J Urol157: 1304-1308, 1997.","DOI":"10.1016\/S0022-5347(01)64958-9"},{"key":"REF9","doi-asserted-by":"crossref","unstructured":"Cai S, Ferguson DG, Martin AF, and Paul RJ.Smooth muscle contractility is modulated by myosin tail-S2-LMM hinge region interaction.Am J Physiol Cell Physiol269: C1126-C1132, 1995.","DOI":"10.1152\/ajpcell.1995.269.5.C1126"},{"key":"REF10","doi-asserted-by":"crossref","unstructured":"Chang S, Hypolite JA, Zderic SA, Wein AJ, Chacko S, and DiSanto ME.Enhanced force generation by corpus cavernosum smooth muscle in rabbits with partial bladder outlet obstruction.J Urol167: 2636-2644, 2002.","DOI":"10.1016\/S0022-5347(05)65050-1"},{"key":"REF11","doi-asserted-by":"crossref","unstructured":"Cher ML, Abernathy BB, McConnell JD, Zimmern PE, and Lin VK.Smooth-muscle myosin heavy-chain isoform expression in bladder-outlet obstruction.World J Urol14: 295-300, 1996.","DOI":"10.1007\/BF00184601"},{"key":"REF12","unstructured":"Chisholm RL, Rushforth AM, Pollenz RS, Kuczmarski ER, and Tafuri SR.Dictyosteliumdiscoideum myosin: isolation and characterization of cDNAs encoding the essential light chain.Mol Cell Biol8: 794-801, 1988."},{"key":"REF13","doi-asserted-by":"crossref","unstructured":"DiSanto ME, Cox RH, Wang Z, and Chacko S.NH2-terminal-inserted myosin II heavy chain is expressed in smooth muscle of small muscular arteries.Am J Physiol Cell Physiol272: C1532-C1542, 1997.","DOI":"10.1152\/ajpcell.1997.272.5.C1532"},{"key":"REF14","doi-asserted-by":"crossref","unstructured":"DiSanto ME, Wang Z, Menon C, Zheng Y, Chacko T, Hypolite J, Broderick G, Wein AJ, and Chacko S.Expression of myosin isoforms in smooth muscle cells in the corpus cavernosum penis.Am J Physiol Cell Physiol275: C976-C987, 1998.","DOI":"10.1152\/ajpcell.1998.275.4.C976"},{"key":"REF15","doi-asserted-by":"crossref","unstructured":"Eddinger TJ, Korwek AA, Meer DP, and Sherwood JJ.Expression of smooth muscle myosin light chain 17 and unloaded shortening in single smooth muscle cells.Am J Physiol Cell Physiol278: C1133-C1142, 2000.","DOI":"10.1152\/ajpcell.2000.278.6.C1133"},{"key":"REF16","doi-asserted-by":"crossref","unstructured":"Eddinger TJand Meer DP.Single rabbit stomach smooth muscle cell myosin heavy chain SMB expression and shortening velocity.Am J Physiol Cell Physiol280: C309-C316, 2001.","DOI":"10.1152\/ajpcell.2001.280.2.C309"},{"key":"REF17","doi-asserted-by":"crossref","unstructured":"Elbadawi A, Yalla SV, and Resnick NM.Structural basis of geriatric voiding dysfunction. IV. Bladder outlet obstruction.J Urol150: 1681-1695, 1993.","DOI":"10.1016\/S0022-5347(17)35869-X"},{"key":"REF18","doi-asserted-by":"crossref","unstructured":"Han Fand Lillard SJ.In-situ sampling and separation of RNA from individual mammalian cells.Anal Chem72: 4073-4079, 2000.","DOI":"10.1021\/ac000428g"},{"key":"REF19","unstructured":"Hartshorne DJ.Physiology of the Gastrointestinal Tract. New York: Raven, 1987, p. 423-482."},{"key":"REF20","doi-asserted-by":"crossref","unstructured":"Hasegawa Yand Morita F.Role of 17-kDa essential light chain isoforms of aorta smooth muscle myosin.J Biochem (Tokyo)111: 804-809, 1992.","DOI":"10.1093\/oxfordjournals.jbchem.a123840"},{"key":"REF21","doi-asserted-by":"crossref","unstructured":"Hasegawa Y, Ueno H, Horie K, and Morita F.Two isoforms of 17-kDa essential light chain of aorta media smooth muscle myosin.J Biochem (Tokyo)103: 15-18, 1988.","DOI":"10.1093\/oxfordjournals.jbchem.a122222"},{"key":"REF22","doi-asserted-by":"crossref","unstructured":"Helper DJ, Lash JA, and Hathaway DR.Distribution of isoelectric variants of the 17,000-dalton myosin light chain in mammalian smooth muscle.J Biol Chem263: 15748-15753, 1988.","DOI":"10.1016\/S0021-9258(19)37651-3"},{"key":"REF22","unstructured":"[Corrigenda.J Biol Chem264: February 1989, p. 2391.]"},{"key":"REF23","doi-asserted-by":"crossref","unstructured":"Huang QQ, Fisher SA, and Brozovich FV.Forced expression of essential myosin light chain isoforms demonstrates their role in smooth muscle force production.J Biol Chem274: 35095-35098, 1999.","DOI":"10.1074\/jbc.274.49.35095"},{"key":"REF24","doi-asserted-by":"crossref","unstructured":"Hypolite JA, Broderick G, and Levin RM.Correlation of contractile function of the rabbit corpus cavernosum with NADH fluorescence.Pharmacology46: 353-361, 1993.","DOI":"10.1159\/000139066"},{"key":"REF25","doi-asserted-by":"crossref","unstructured":"Hypolite JA, DiSanto ME, Zheng YM, Chang SH, Wein AJ, and Chacko S.Regional variation in myosin isoforms and phosphorylation at the resting tone in urinary bladder smooth muscle.Am J Physiol Cell Physiol280: C254-C264, 2001.","DOI":"10.1152\/ajpcell.2001.280.2.C254"},{"key":"REF26","doi-asserted-by":"crossref","unstructured":"Kato K, Monson FC, Longhurst PA, Wein AJ, Haugaard N, and Levin RM.The functional effects of long-term outlet obstruction on the rabbit urinary bladder.J Urol143: 600-606, 1990.","DOI":"10.1016\/S0022-5347(17)40038-3"},{"key":"REF27","doi-asserted-by":"crossref","unstructured":"Kato K, Wein AJ, Radzinski C, Longhurst PA, McGuire EJ, Miller LF, Elbadawi A, and Levin RM.Short term functional effects of bladder outlet obstruction in the cat.J Urol143: 1020-1025, 1990.","DOI":"10.1016\/S0022-5347(17)40175-3"},{"key":"REF28","unstructured":"Kawano Mand Mori N.The significance of prostacyclin produced by pregnant rat myometrium: the relationship between myometrial prostacyclin producing activity and passive stretch of myometrium by growing conceptus.Prostaglandins35: 305-325, 1988."},{"key":"REF29","doi-asserted-by":"crossref","unstructured":"Kelley CA, Takahashi M, Yu JH, and Adelstein RS.An insert of seven amino acids confers functional differences between smooth muscle myosins from the intestines and vasculature.J Biol Chem268: 12848-12854, 1993.","DOI":"10.1016\/S0021-9258(18)31465-0"},{"key":"REF30","doi-asserted-by":"crossref","unstructured":"Lenz S, Lohse P, Seidel U, and Arnold HH.The alkali light chains of human smooth and nonmuscle myosins are encoded by a single gene. Tissue-specific expression by alternative splicing pathways.J Biol Chem264: 9009-9015, 1989.","DOI":"10.1016\/S0021-9258(18)81895-6"},{"key":"REF31","doi-asserted-by":"crossref","unstructured":"Levin RM, Hypolite J, and Broderick GA.Comparative studies on intracellular calcium and NADH fluorescence of the rabbit corpus cavernosum.Neurourol Urodyn13: 609-618, 1994.","DOI":"10.1002\/nau.1930130517"},{"key":"REF32","doi-asserted-by":"crossref","unstructured":"Levin RM, Longhurst PA, Monson FC, Kato K, and Wein AJ.Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder.Prostate Suppl3: 9-26, 1990.","DOI":"10.1002\/pros.2990170503"},{"key":"REF33","doi-asserted-by":"crossref","unstructured":"Levin RM, Memberg W, Ruggieri MR, and Wein AJ.Functional effects of in vitro obstruction on the rabbit urinary bladder.J Urol135: 847-851, 1986.","DOI":"10.1016\/S0022-5347(17)45874-5"},{"key":"REF34","unstructured":"Levin RM, Ruggieri MR, Velagapudi S, Gordon D, Altman B, and Wein AJ.Relevance of spontaneous activity to urinary bladder function: an in vitro and in vivo study.J Urol136: 517-521, 1986."},{"key":"REF35","unstructured":"Levin RM, Yu HJ, Kim KB, Longhurst PA, Wein AJ, and Damaser MS.Etiology of bladder dysfunction secondary to partial outlet obstruction. Calcium disregulation in bladder power generation and the ability to perform work.Scand J Urol Nephrol Suppl184: 43-50, 1997."},{"key":"REF36","doi-asserted-by":"crossref","unstructured":"Lin AT, Yang CH, Chen CJ, Chen MT, Chiang H, and Chang LS.Correlation of contractile function and passive properties of rabbit urinary bladder subjected to outlet obstruction\u2014an in vitro whole bladder study.J Urol148: 944-948, 1992.","DOI":"10.1016\/S0022-5347(17)36783-6"},{"key":"REF37","doi-asserted-by":"crossref","unstructured":"Malkowicz SB, Wein AJ, Elbadawi A, Van Arsdalen K, Ruggieri MR, and Levin RM.Acute biochemical and functional alterations in the partially obstructed rabbit urinary bladder.J Urol136: 1324-1329, 1986.","DOI":"10.1016\/S0022-5347(17)45331-6"},{"key":"REF38","doi-asserted-by":"crossref","unstructured":"Malmqvist Uand Arner A.Correlation between isoform composition of the 17 kDa myosin light chain and maximal shortening velocity in smooth muscle.Pfl\u00fcgers Arch418:523-530, 1991.","DOI":"10.1007\/BF00370566"},{"key":"REF39","doi-asserted-by":"crossref","unstructured":"Matthew JD, Khromov AS, Trybus KM, Somlyo AP, and Somlyo AV.Myosin essential light chain isoforms modulate the velocity of shortening propelled by nonphosphorylated cross-bridges.J Biol Chem273: 31289-31296, 1998.","DOI":"10.1074\/jbc.273.47.31289"},{"key":"REF40","doi-asserted-by":"crossref","unstructured":"Morano I, Koehlen S, Haase H, Erb G, Baltas LG, Rimbach S, Wallwiener D, and Bastert G.Alternative splicing and cycling kinetics of myosin change during hypertrophy of human smooth muscle cells.J Cell Biochem64: 171-181, 1997.","DOI":"10.1002\/(SICI)1097-4644(199702)64:2<171::AID-JCB1>3.0.CO;2-U"},{"key":"REF41","unstructured":"Mostwin JL.Pathophysiology: the varieties of bladder overactivity.Urology60: 22-26, 2002."},{"key":"REF42","doi-asserted-by":"crossref","unstructured":"Nabeshima Y, Nonomura Y, and Fujii-Kuriyama Y.Nonmuscle and smooth muscle myosin light chain mRNAs are generated from a single gene by the tissue-specific alternative RNA splicing.J Biol Chem262: 10608-10612, 1987.","DOI":"10.1016\/S0021-9258(18)61006-3"},{"key":"REF43","doi-asserted-by":"crossref","unstructured":"O'Farrell PH.High resolution two-dimensional electrophoresis of proteins.J Biol Chem250: 4007-4021, 1975.","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"REF44","unstructured":"Ogura M, Agata Y, Watanabe K, McCormick RM, Hamaguchi Y, Aso Y, and Mitsuhashi M.RNA chip: quality assessment of RNA by microchannel linear gel electrophoresis in injection-molded plastic chips.Clin Chem44: 2249-2255, 1998."},{"key":"REF45","doi-asserted-by":"crossref","unstructured":"Osada T, Uehara H, Kim H, and Ikai A.mRNA analysis of single living cells.J Nanobiotechnol1: 2-8. 2003.","DOI":"10.1186\/1477-3155-1-2"},{"key":"REF46","doi-asserted-by":"crossref","unstructured":"Paterson BMand Bishop JO.Changes in the mRNA population of chick myoblasts during myogenesis in vitro.Cell12: 751-765, 1977.","DOI":"10.1016\/0092-8674(77)90275-6"},{"key":"REF47","unstructured":"Poulsen AL, Schou J, Puggaard L, Torp-Pedersen S, and Nordling J.Prostatic enlargement, symptomatology and pressure\/flow evaluation: interrelations in patients with symptomatic BPH.Scand J Urol Nephrol Suppl157: 67-73, 1994."},{"key":"REF48","unstructured":"Pyne GJ, Lalli MJ, Martin AF, and Paul RJ.Role of C-terminal myosin heavy chain isoforms in smooth muscle function: evidence from mice overexpressing SMaSM1 (Abstract).Biophys J82: 1735, 2002."},{"key":"REF49","doi-asserted-by":"crossref","unstructured":"Quevillon-Cheruel S, Janmot C, Nozais M, Lompre AM, and Bechet JJ.Functional regions in the essential light chain of smooth muscle myosin as revealed by the mutagenesis approach.Eur J Biochem267: 6151-6157, 2000.","DOI":"10.1046\/j.1432-1327.2000.01668.x"},{"key":"REF50","doi-asserted-by":"crossref","unstructured":"Rovner AS, Fagnant PM, Lowey S, and Trybus KM.The carboxyl-terminal isoforms of smooth muscle myosin heavy chain determine thick filament assembly properties.J Cell Biol156: 113-123, 2002.","DOI":"10.1083\/jcb.200107131"},{"key":"REF51","doi-asserted-by":"crossref","unstructured":"Rovner AS, Freyzon Y, and Trybus KM.An insert in the motor domain determines the functional properties of expressed smooth muscle myosin isoforms.J Muscle Res Cell Motil18: 103-110, 1997.","DOI":"10.1023\/A:1018689102122"},{"key":"REF52","doi-asserted-by":"crossref","unstructured":"Sarioglu Y, Yildirim S, Utkan T, Yildirim MK, and Uma S.Evidence of relaxant effect of omeprazole in rabbit corpus cavernosum in vitro.Life Sci66: 1411-1421, 2000.","DOI":"10.1016\/S0024-3205(00)00452-5"},{"key":"REF53","doi-asserted-by":"crossref","unstructured":"Sherwood JJand Eddinger TJ.Shortening velocity and myosin heavy- and light-chain isoform mRNA in rabbit arterial smooth muscle cells.Am J Physiol Cell Physiol282: C1093-C1102, 2002.","DOI":"10.1152\/ajpcell.00307.2001"},{"key":"REF54","unstructured":"Siegman MJ, Butler TM, Mooers SU, Trinkle-Mulcahy L, Narayan S, Adam L, Chacko S, Haase H, and Morano I.Hypertrophy of colonic smooth muscle: contractile proteins, shortening velocity, and regulation.Am J Physiol Gastrointest Liver Physiol272: G1571-G1580, 1997."},{"key":"REF55","unstructured":"Stein R, Hutcheson JC, Krasnopolsky L, Canning DA, Carr MC, and Zderic SA.The decompensated detrusor V: molecular correlates of bladder function after reversal of experimental outlet obstruction.J Urol166: 651-657, 2001."},{"key":"REF56","doi-asserted-by":"crossref","unstructured":"Sweeney HL, Rosenfeld SS, Brown F, Faust L, Smith J, Xing J, Stein LA, and Sellers JR.Kinetic tuning of myosin via a flexible loop adjacent to the nucleotide binding pocket.J Biol Chem273: 6262-6270, 1998.","DOI":"10.1074\/jbc.273.11.6262"},{"key":"REF57","doi-asserted-by":"crossref","unstructured":"Thornbury KD.Tonic and phasic activity in smooth muscle.Ir J Med Sci168: 201-207, 1999.","DOI":"10.1007\/BF02945854"},{"key":"REF58","doi-asserted-by":"crossref","unstructured":"Tse V, Wills E, Szonyi G, and Khadra MH.The application of ultrastructural studies in the diagnosis of bladder dysfunction in a clinical setting.J Urol163: 535-539, 2000.","DOI":"10.1016\/S0022-5347(05)67919-0"},{"key":"REF59","doi-asserted-by":"crossref","unstructured":"Upadhya A, Samuel M, Cox RH, Bagshaw RJ, and Chacko S.Characteristics of arterial myosin in experimental renal hypertension in the dog.Hypertension21: 624-631, 1993.","DOI":"10.1161\/01.HYP.21.5.624"},{"key":"REF60","unstructured":"Wang ZE, Gopalakurup SK, Levin RM, and Chacko S.Expression of smooth muscle myosin isoforms in urinary bladder smooth muscle during hypertrophy and regression.Lab Invest73: 244-251, 1995."},{"key":"REF61","doi-asserted-by":"crossref","unstructured":"Zheng Y, Weber WT, Wang S, Wein AJ, Zderic SA, Chacko S, and DiSanto ME.Generation of a cell line with smooth muscle phenotype from hypertrophied urinary bladder.Am J Physiol Cell Physiol283: C373-C382, 2002.","DOI":"10.1152\/ajpcell.00002.2002"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00513.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T09:54:25Z","timestamp":1746006865000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00513.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":62,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1152\/ajpcell.00513.2002"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00513.2002","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}