{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:16:49Z","timestamp":1773418609635,"version":"3.50.1"},"reference-count":37,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p> One of the unanswered questions in muscle hypertrophy is how new contractile units are inserted into a stable existing cytoskeletal meshwork. Regulation of actin capping by CapZ may play a role in remodeling processes, therefore, CapZ dynamics are determined during rapid growth of cardiac cells in vitro. Neonatal rat ventricular myocytes were infected with adenovirus expressing green fluorescent protein-CapZ \u03b21 and responded normally to hypertrophic stimuli. CapZ dynamics were analyzed by fluorescence recovery after photobleaching in cultured myocytes treated with endothelin-1 (100 nM) or phenylephrine (10 \u03bcM). Recovery by 30 s was greater with endothelin treatment. Analysis 30 min postbleach showed CapZ-infected cells treated with endothelin recovered more completely than controls (77 \u00b1 9% vs. 50 \u00b1 6%, P &lt; 0.001). Similar results were found with phenylephrine (77 \u00b1 5%, P &lt; 0.05). A potential mechanism for phosphatidylinositol bisphosphate (PIP2) mediation of increased CapZ exchange in endothelin- and phenylephrine-treated cells was tested. PIP2 sequestration with neomycin (500 \u03bcM) blocked both endothelin- (43 \u00b1 6%, P &lt; 0.001) and phenylephrine (36 \u00b1 4%, P &lt; 0.001)-mediated recovery. The protein kinase C inhibitor chelerythrine chloride (10 \u03bcM) also blocked endothelin- (53 \u00b1 10%, P &lt; 0.001) and phenylephrine (42 \u00b1 3%, P &lt; 0.001)-mediated recovery. This study demonstrates for the first time that endothelin and phenylephrine alter CapZ dynamics through PIP2- and PKC-dependent pathways, which might destabilize the existing framework and permit sarcomeric remodelling to proceed. <\/jats:p>","DOI":"10.1152\/ajpcell.00544.2008","type":"journal-article","created":{"date-parts":[[2009,3,19]],"date-time":"2009-03-19T02:05:25Z","timestamp":1237428325000},"page":"C1034-C1039","source":"Crossref","is-referenced-by-count":37,"title":["CapZ dynamics are altered by endothelin-1 and phenylephrine via PIP2- and PKC-dependent mechanisms"],"prefix":"10.1152","volume":"296","author":[{"given":"Thomas J.","family":"Hartman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jody L.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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