{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T05:01:14Z","timestamp":1780722074796,"version":"3.54.1"},"reference-count":27,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2002,10,1]]},"abstract":"<jats:p> Muscular K uptake depends on skeletal muscle Na-K-ATPase concentration and activity. Reduced K uptake is observed in vitro in K-depleted rats. We evaluated skeletal muscle K clearance capacity in vivo in rats K depleted for 14 days. [<jats:sup>3<\/jats:sup>H]ouabain binding, \u03b1<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>2<\/jats:sub> Na-K-ATPase isoform abundance, and K, Na, and Mg content were measured in skeletal muscles. Skeletal muscle K, Na, and Mg and plasma K were measured in relation to intravenous KCl infusion that continued until animals died, i.e., maximum KCl dose was administered. In soleus, extensor digitorum longus (EDL), and gastrocnemius muscles K depletion significantly reduced K content by 18%, 15%, and 19%, [<jats:sup>3<\/jats:sup>H]ouabain binding by 36%, 41%, and 68%, and \u03b1<jats:sub>2<\/jats:sub> isoform abundance by 34%, 44%, and 70%, respectively. No significant change was observed in \u03b1<jats:sub>1<\/jats:sub> isoform abundance. In EDL and gastrocnemius muscles K depletion significantly increased Na (48% and 59%) and Mg (10% and 17%) content, but only tendencies to increase were observed in soleus muscle. K-depleted rats tolerated up to a fourfold higher KCl dose. This was associated with a reduced rate of increase in plasma K and increases in soleus, EDL, and gastrocnemius muscle K of 56%, 42%, and 41%, respectively, but only tendencies to increase in controls. However, whereas K uptake was highest in K-depleted animals, the K uptake rate was highest in controls. In vivo K depletion is associated with markedly increased K tolerance and K clearance despite significantly reduced skeletal muscle Na-K-ATPase concentration. The concern of an increased risk for K intoxication during K repletion seems unwarranted. <\/jats:p>","DOI":"10.1152\/ajpcell.00588.2001","type":"journal-article","created":{"date-parts":[[2013,5,14]],"date-time":"2013-05-14T00:10:48Z","timestamp":1368490248000},"page":"C1163-C1170","source":"Crossref","is-referenced-by-count":18,"title":["Potassium depletion increases potassium clearance capacity  in skeletal muscles in vivo during acute repletion"],"prefix":"10.1152","volume":"283","author":[{"given":"Henning","family":"Bundgaard","sequence":"first","affiliation":[{"name":"Medical Department, The Heart Centre, Rigshospitalet, National University Hospital, University of Copenhagen, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keld","family":"Kjeldsen","sequence":"additional","affiliation":[{"name":"Medical Department, The Heart Centre, Rigshospitalet, National University Hospital, University of Copenhagen, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0764"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.82.4.1136"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.1.F95"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1986.66.3.542"},{"key":"B5","first-page":"24","volume":"11","author":"Clausen T.","year":"1996","journal-title":"News Physiol Sci"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1989.1"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016608"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199307000-00001"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201X.1996.208000.x"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.2.C457"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.296.6620.455"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.4.C925"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201X.2001.00910.x"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1042\/bj2400725"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19860133"},{"key":"B17","first-page":"55","volume":"6","author":"Kjeldsen K","year":"1987","journal-title":"Magnesium"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1984.tb07488.x"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)91996-2"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.3.C675"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.1.85"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/293739a0"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1987.63.2.765"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(88)56004-4"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.276.6.C1411"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.51.32653"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.324.7332.269"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00588.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:12:57Z","timestamp":1718651577000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00588.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10,1]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,10,1]]}},"alternative-id":["10.1152\/ajpcell.00588.2001"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00588.2001","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10,1]]}}}