{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:22:47Z","timestamp":1791426167875,"version":"4.3.3"},"reference-count":46,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p>\n                    Bone is a dynamic tissue that is continuously being remodeled throughout life. Specialized cells called osteoclasts transiently break down old bone (resorption process) at multiple sites as other cells known as osteoblasts are replacing it with new tissue (bone formation). Usually, both resorption and formation processes are in balance and thereby maintain skeletal strength and integrity. This equilibrium is assured by the coordination of proliferation, migration, differentiation, and secretory functions of the osteoblasts, which are essential for adequate formation and resorption processes. Disturbances of this equilibrium may lead to decreased bone mass (osteoporosis), increased bone fragility, and susceptibility to fractures. Epidemiological studies have linked insufficient dietary magnesium (Mg\n                    <jats:sup>2+<\/jats:sup>\n                    ) intake in humans with low bone mass and osteoporosis. Here, we investigated the roles of Mg\n                    <jats:sup>2+<\/jats:sup>\n                    and melastatin-like transient receptor potential 7 (TRPM7), known as Mg\n                    <jats:sup>2+<\/jats:sup>\n                    channels, in human osteoblast cell proliferation and migration induced by platelet-derived growth factor (PDGF), which has been involved in the bone remodeling process. PDGF promoted an influx of Mg\n                    <jats:sup>2+<\/jats:sup>\n                    , enhanced cell migration, and stimulated the gene expression of TRPM7 channels in human osteoblast MG-63 cells. The stimulation of osteoblast proliferation and migration by PDGF was significantly reduced under culture conditions of low extracellular Mg\n                    <jats:sup>2+<\/jats:sup>\n                    concentrations. Silencing TRPM7 expression in osteoblasts by specific small interfering RNA prevented the induction by PDGF of Mg\n                    <jats:sup>2+<\/jats:sup>\n                    influx, proliferation, and migration. Our results indicate that extracellular Mg\n                    <jats:sup>2+<\/jats:sup>\n                    and TRPM7 are important for PDGF-induced proliferation and migration of human osteoblasts. Thus Mg\n                    <jats:sup>2+<\/jats:sup>\n                    deficiency, a common condition among the general population, may be associated with altered osteoblast functions leading to inadequate bone formation and the development of osteoporosis.\n                  <\/jats:p>","DOI":"10.1152\/ajpcell.00614.2008","type":"journal-article","created":{"date-parts":[[2009,5,27]],"date-time":"2009-05-27T22:14:16Z","timestamp":1243462456000},"page":"C360-C368","source":"Crossref","is-referenced-by-count":123,"title":["Importance of melastatin-like transient receptor potential 7 and magnesium in the stimulation of osteoblast proliferation and migration by platelet-derived growth factor"],"prefix":"10.1152","volume":"297","author":[{"given":"Elie","family":"Abed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Moreau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"R1","doi-asserted-by":"crossref","unstructured":"Abed E\n                      , Moreau R.\n                      Importance of melastatin-like transient receptor potential 7 and cations (magnesium, calcium) in human osteoblast-like cell proliferation.\n                      Cell Prolif\n                      40\n                      : 849\u2013865, 2007.","DOI":"10.1111\/j.1365-2184.2007.00476.x"},{"key":"R2","doi-asserted-by":"crossref","unstructured":"Aubin JE\n                      .\n                      Advances in the osteoblast lineage.\n                      Biochem Cell Biol\n                      76\n                      : 899\u2013910, 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synthesis in osteoblast-enriched cultures from fetal rat parietal bone.\n                      Endocrinology\n                      125\n                      : 13\u201319, 1989.","DOI":"10.1210\/endo-125-1-13"},{"key":"R6","doi-asserted-by":"crossref","unstructured":"Chubanov V\n                      , Waldegger S, Schnitzler M, Vitzthum H, Sassen MC, Seyberth HW, Konrad M, Gudermann T.\n                      Disruption of TRPM6\/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia.\n                      Proc Natl Acad Sci USA\n                      101\n                      : 2894\u20132899, 2004.","DOI":"10.1073\/pnas.0305252101"},{"key":"R7","doi-asserted-by":"crossref","unstructured":"Clapham DE\n                      , Runnels LW, Strubing C.\n                      The TRP ion channel family.\n                      Nat Rev Neurosci\n                      2\n                      : 387\u2013396, 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Role of cellular magnesium in health and human disease.\n                      Front Biosci\n                      9\n                      : 262\u2013276, 2004.","DOI":"10.2741\/1223"},{"key":"R21","doi-asserted-by":"crossref","unstructured":"Mackie E\n                      .\n                      Osteoblasts: novel roles in orchestration of skeletal architecture.\n                      Int J Biochem Cell Biol\n                      35\n                      : 1301\u20131305, 2003.","DOI":"10.1016\/S1357-2725(03)00107-9"},{"key":"R22","doi-asserted-by":"crossref","unstructured":"Maier JA\n                      , Malpuech-Brugere C, Zimowska W, Rayssiguier Y, Mazur A.\n                      Low magnesium promotes endothelial cell dysfunction: implications for atherosclerosis, inflammation and thrombosis.\n                      Biochim Biophys Acta\n                      1689\n                      : 13\u201321, 2004.","DOI":"10.1016\/j.bbadis.2004.01.002"},{"key":"R23","doi-asserted-by":"crossref","unstructured":"Mehrotra M\n                      , Krane SM, Walters K, Pilbeam C.\n                      Differential regulation of platelet-derived growth factor stimulated migration and proliferation in osteoblastic cells.\n                      J Cell Biochem\n                      93\n                      : 741\u2013752, 2004.","DOI":"10.1002\/jcb.20138"},{"key":"R24","doi-asserted-by":"crossref","unstructured":"Mehrotra M\n                      , Krane SM, Walters K, Pilbeam C.\n                      Differential regulation of platelet-derived growth factor stimulated migration and proliferation in osteoblastic cells.\n                      J Cell Biochem\n                      93\n                      : 741\u2013752, 2004.","DOI":"10.1002\/jcb.20138"},{"key":"R25","doi-asserted-by":"crossref","unstructured":"Minke B\n                      , Cook B.\n                      TRP channel proteins and signal transduction.\n                      Physiol Rev\n                      82\n                      : 429\u2013472, 2002.","DOI":"10.1152\/physrev.00001.2002"},{"key":"R26","doi-asserted-by":"crossref","unstructured":"Montell C\n                      , Birnbaumer L, Flockerzi V.\n                      The TRP channels, a remarkably functional family.\n                      Cell\n                      108\n                      : 595\u2013598, 2002.","DOI":"10.1016\/S0092-8674(02)00670-0"},{"key":"R27","doi-asserted-by":"crossref","unstructured":"Nadler MJ\n                      , Hermosura MC, Inabe K, Perraud AL, Zhu Q, Stokes AJ, Kurosaki T, Kinet JP, Penner R, Scharenberg AM, Fleig A.\n                      LTRPC7 is a Mg.ATP-regulated divalent cation channel required for cell viability.\n                      Nature\n                      411\n                      : 590\u2013595, 2001.","DOI":"10.1038\/35079092"},{"key":"R28","doi-asserted-by":"crossref","unstructured":"Nakajima T\n                      , Iwasawa K, Hazama H, Asano M, Okuda Y, Omata M.\n                      Extracellular Mg\n                      2+\n                      inhibits receptor-mediated Ca(\n                      2+\n                      )-permeable non-selective cation currents in aortic smooth muscle cells.\n                      Eur J Pharmacol\n                      320\n                      : 81\u201386, 1997.","DOI":"10.1016\/S0014-2999(96)00873-4"},{"key":"R29","doi-asserted-by":"crossref","unstructured":"Pfeilschifter J\n                      , Oechsner M, Naumann A, Gronwald RG, Minne HW, Ziegler R.\n                      Stimulation of bone matrix apposition in vitro by local growth factors: a comparison between insulin-like growth factor I, platelet-derived growth factor, and transforming growth factor beta.\n                      Endocrinology\n                      127\n                      : 69\u201375, 1990.","DOI":"10.1210\/endo-127-1-69"},{"key":"R30","unstructured":"Rubin H\n                      .\n                      The membrane, magnesium, mitosis (MMM) model of cell proliferation control.\n                      Magnes Res\n                      18\n                      : 268\u2013274, 2005."},{"key":"R31","doi-asserted-by":"crossref","unstructured":"Rude RK\n                      , Gruber HE.\n                      Magnesium deficiency and osteoporosis: animal and human observations.\n                      J Nutr Biochem\n                      15\n                      : 710\u2013716, 2004.","DOI":"10.1016\/j.jnutbio.2004.08.001"},{"key":"R32","doi-asserted-by":"crossref","unstructured":"Rude RK\n                      , Gruber HE, Norton HJ, Wei LY, Frausto A, Mills BG.\n                      Bone loss induced by dietary magnesium reduction to 10% of the nutrient requirement in rats is associated with increased release of substance P and tumor necrosis factor-alpha.\n                      J Nutr\n                      134\n                      : 79\u201385, 2004.","DOI":"10.1093\/jn\/134.1.79"},{"key":"R33","unstructured":"Rude RK\n                      , Kirchen ME, Gruber HE, Meyer MH, Luck JS, Crawford DL.\n                      Magnesium deficiency-induced osteoporosis in the rat: uncoupling of bone formation and bone resorption.\n                      Magnes Res\n                      12\n                      : 257\u2013267, 1999."},{"key":"R34","doi-asserted-by":"crossref","unstructured":"Sahni J\n                      , Nelson B, Scharenberg AM.\n                      SLC41A2 encodes a plasma-membrane Mg\n                      2+\n                      transporter.\n                      Biochem J\n                      401\n                      : 505\u2013513, 2007.","DOI":"10.1042\/BJ20060673"},{"key":"R35","unstructured":"Schmitz C\n                      , Deason F, Perraud AL.\n                      Molecular components of vertebrate Mg\n                      2+\n                      -homeostasis regulation.\n                      Magnes Res\n                      20\n                      : 6\u201318, 2007."},{"key":"R36","doi-asserted-by":"crossref","unstructured":"Schmitz C\n                      , Dorovkov MV, Zhao X, Davenport BJ, Ryazanov AG, Perraud AL.\n                      The channel kinases TRPM6 and TRPM7 are functionally nonredundant.\n                      J Biol Chem\n                      280\n                      : 37763\u201337771, 2005.","DOI":"10.1074\/jbc.M509175200"},{"key":"R37","doi-asserted-by":"crossref","unstructured":"Schmitz C\n                      , Perraud AL, Johnson CO, Inabe K, Smith MK, Penner R, Kurosaki T, Fleig A, Scharenberg AM.\n                      Regulation of vertebrate cellular Mg\n                      2+\n                      homeostasis by TRPM7.\n                      Cell\n                      114\n                      : 191\u2013200, 2003.","DOI":"10.1016\/S0092-8674(03)00556-7"},{"key":"R38","doi-asserted-by":"crossref","unstructured":"Small JV\n                      , Kaverina I, Krylyshkina O, Rottner K.\n                      Cytoskeleton cross-talk during cell motility.\n                      FEBS Lett\n                      452\n                      : 96\u201399, 1999.","DOI":"10.1016\/S0014-5793(99)00530-X"},{"key":"R39","doi-asserted-by":"crossref","unstructured":"Su LT\n                      , Agapito MA, Li M, Simonson WT, Huttenlocher A, Habas R, Yue L, Runnels LW.\n                      TRPM7 regulates cell adhesion by controlling the calcium-dependent protease calpain.\n                      J Biol Chem\n                      281\n                      : 11260\u201311270, 2006.","DOI":"10.1074\/jbc.M512885200"},{"key":"R40","doi-asserted-by":"crossref","unstructured":"Sugden EA\n                      , Nihei T.\n                      The effects of calcium and magnesium ions on the adenosine triphosphatase and inosine triphosphatase activities of myosin A.\n                      Biochem J\n                      113\n                      : 821\u2013827, 1969.","DOI":"10.1042\/bj1130821"},{"key":"R41","doi-asserted-by":"crossref","unstructured":"Tanaka H\n                      , Wakisaka A, Ogasa H, Kawai S, Liang CT.\n                      Effect of IGF-I and PDGF administered in vivo on the expression of osteoblast-related genes in old rats.\n                      J Endocrinol\n                      174\n                      : 63\u201370, 2002.","DOI":"10.1677\/joe.0.1740063"},{"key":"R42","doi-asserted-by":"crossref","unstructured":"Touyz RM\n                      .\n                      Transient receptor potential melastatin 6 and 7 channels, magnesium transport, and vascular biology: implications in hypertension.\n                      Am J Physiol Heart Circ Physiol\n                      294\n                      : H1103\u2013H1118, 2008.","DOI":"10.1152\/ajpheart.00903.2007"},{"key":"R43","doi-asserted-by":"crossref","unstructured":"Verive MJ\n                      , Irazuzta J, Steinhart CM, Orlowski JP, Jaimovich DG.\n                      Evaluating the frequency rate of hypomagnesemia in critically ill pediatric patients by using multiple regression analysis and a computer-based neural network.\n                      Crit Care Med\n                      28\n                      : 3534\u20133539, 2000.","DOI":"10.1097\/00003246-200010000-00031"},{"key":"R44","unstructured":"Vihinen P\n                      , Riikonen T, Laine A, Heino J.\n                      Integrin alpha 2 beta 1 in tumorigenic human osteosarcoma cell lines regulates cell adhesion, migration, and invasion by interaction with type I collagen.\n                      Cell Growth Differ\n                      7\n                      : 439\u2013447, 1996."},{"key":"R45","doi-asserted-by":"crossref","unstructured":"Wykes RC\n                      , Lee M, Duffy SM, Yang W, Seward EP, Bradding P.\n                      Functional transient receptor potential melastatin 7 channels are critical for human mast cell survival.\n                      J Immunol\n                      179\n                      : 4045\u20134052, 2007.","DOI":"10.4049\/jimmunol.179.6.4045"},{"key":"R46","doi-asserted-by":"crossref","unstructured":"Zhang A\n                      , Cheng TP, Altura BM.\n                      Magnesium regulates intracellular free ionized calcium concentration and cell geometry in vascular smooth muscle cells.\n                      Biochim Biophys Acta\n                      1134\n                      : 25\u201329, 1992.","DOI":"10.1016\/0167-4889(92)90024-6"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00614.2008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T13:12:34Z","timestamp":1718629954000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00614.2008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1152\/ajpcell.00614.2008"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00614.2008","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]}}}