{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T12:08:40Z","timestamp":1792757320852,"version":"4.5.0"},"reference-count":74,"publisher":"American Physiological Society","issue":"5","funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01GI0925"],"award-info":[{"award-number":["01GI0925"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010215","name":"Deutsche Diabetes Gesellschaft","doi-asserted-by":"publisher","award":["Allgemeine Projektf&#x00F6"],"award-info":[{"award-number":["Allgemeine Projektf&#x00F6"]}],"id":[{"id":"10.13039\/501100010215","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010215","name":"Deutsche Diabetes Gesellschaft","doi-asserted-by":"publisher","award":["rderung"],"award-info":[{"award-number":["rderung"]}],"id":[{"id":"10.13039\/501100010215","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2024,5,1]]},"abstract":"<jats:p>Human skeletal muscle models are highly valuable to study how exercise prevents type 2 diabetes without invasive biopsies. Current models did not fully recapitulate the function of skeletal muscle especially during exercise. By supplementing insulin-like growth factor 1 (IGF1), the authors developed a functional human skeletal muscle model characterized by inducible contractility and increased oxidative and insulin-sensitive metabolism. The novel protocol overcomes the limitations of previous standards and enables investigation of exercise on a molecular level.<\/jats:p>","DOI":"10.1152\/ajpcell.00654.2023","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T04:01:45Z","timestamp":1709524905000},"page":"C1462-C1481","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype"],"prefix":"10.1152","volume":"326","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-1624","authenticated-orcid":false,"given":"Simon I.","family":"Dreher","sequence":"first","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Grubba","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"von Toerne","sequence":"additional","affiliation":[{"name":"Metabolomics and Proteomics Core Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Moruzzi","sequence":"additional","affiliation":[{"name":"NMI Natural and Medical Sciences Institute at the University of T\u00fcbingen, Reutlingen, Germany"},{"name":"Department for Microphysiological Systems, Institute of Biomedical Engineering, Faculty of Medicine, Eberhard Karls University T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Maurer","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Goj","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"German Center for Diabetes Research (DZD), Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas L.","family":"Birkenfeld","sequence":"additional","affiliation":[{"name":"German Center for Diabetes Research (DZD), Neuherberg, Germany"},{"name":"Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum M\u00fcnchen, University of T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"Department of Internal Medicine IV, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5732-2287","authenticated-orcid":false,"given":"Andreas","family":"Peter","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"German Center for Diabetes Research (DZD), Neuherberg, Germany"},{"name":"Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum M\u00fcnchen, University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Loskill","sequence":"additional","affiliation":[{"name":"NMI Natural and Medical Sciences Institute at the University of T\u00fcbingen, Reutlingen, Germany"},{"name":"Department for Microphysiological Systems, Institute of Biomedical Engineering, Faculty of Medicine, Eberhard Karls University T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1630-6827","authenticated-orcid":false,"given":"Stefanie M.","family":"Hauck","sequence":"additional","affiliation":[{"name":"Metabolomics and Proteomics Core Helmholtz Center Munich, German Research Center for Environmental Health, Neuherberg, Germany"},{"name":"German Center for Diabetes Research (DZD), Neuherberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0358-776X","authenticated-orcid":false,"given":"Cora","family":"Weigert","sequence":"additional","affiliation":[{"name":"Department for Diagnostic Laboratory Medicine, Institute for Clinical Chemistry and Pathobiochemistry, University Hospital T\u00fcbingen, T\u00fcbingen, Germany"},{"name":"German Center for Diabetes Research (DZD), Neuherberg, Germany"},{"name":"Institute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum M\u00fcnchen, University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2007.01783.x"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-007-0910-y"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.28.1.108"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2012.12.012"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00038.2012"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20210185"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.2337\/db16-1327"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1113\/EP085319"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1172\/jci110399"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1210\/endrev\/bnaa016"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029793"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.06.004"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-021-00372-0"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2021.101281"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.1c01536"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1042\/ETLS20190061"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510x(71)90037-2"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.25.1.58"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510x(77)90018-1"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1007\/BF00381893"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(91)90375-5"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.9.5623"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.2337\/diab.44.8.936"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18658-3"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1039\/C3BM60166H"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-013-1655-1"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-012-2454-z"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033203"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2010.09.014"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116005"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.49.6.992"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2003.10.034"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2004.05.023"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3118"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-020-00709-1"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1430505"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.3390\/cells10123443"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.2337\/db15-1723"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgaa571"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEA.2019.0002"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M115.052183"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1322"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1021\/pr4006958"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1186\/2044-5040-1-4"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0203-87"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-013-1330-4"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00041.2007"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00579.2003"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.05.032"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.10.007"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/s1359-6101(96)00029-9"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03371"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.093906"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.3390\/cells10071671"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2016.2536"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.7.2090"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/s0006-2952(02)01509-5"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.1080\/17461391.2016.1185164"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2003.00247.x"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2011.02.007"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00408.2013"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abo3192"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00043.2013"},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704817200"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1038\/nature00904"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.49.7.1092"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.2000.278.5.E910"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1530\/JME-17-0311"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.3390\/cells9091970"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-012-9751-7"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1021\/bp060121o"},{"key":"B73","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2018.08.058"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-020-00242-y"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.77204"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00654.2023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T10:43:47Z","timestamp":1718621027000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00654.2023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,1]]},"references-count":74,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5,1]]}},"alternative-id":["10.1152\/ajpcell.00654.2023"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00654.2023","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.06.12.544344","asserted-by":"object"}]},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,1]]},"assertion":[{"value":"2023-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-26","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}