{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T19:45:43Z","timestamp":1776973543190,"version":"3.51.4"},"reference-count":58,"publisher":"American Physiological Society","issue":"4","funder":[{"DOI":"10.13039\/501100001592","name":"Higher Education Authority","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001592","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.physiology.org"],"crossmark-restriction":true},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[2026,4,1]]},"abstract":"<jats:p>Cholinergic tone is elevated in airway smooth muscle (ASM) of patients with COPD and asthma. Here, we report that M2R-dependent contractions of ASM were inhibited by activators of Kv7.4 and Kv7.5 channels. The cholinergic agonist, carbachol (CCh), suppressed Kv7 currents recorded from HEK293 cells co-expressing M2Rs and Kv7.4 or Kv7.5. These data suggest that the bronchoconstrictor effects of ACh involve M2R-mediated inhibition of Kv7.4 and Kv7.5 channels.<\/jats:p>","DOI":"10.1152\/ajpcell.00752.2025","type":"journal-article","created":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T11:41:09Z","timestamp":1772710869000},"page":"C926-C942","update-policy":"https:\/\/doi.org\/10.1152\/aps-crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["M2 muscarinic receptor-dependent contractions of airway smooth muscle are mediated by inhibition of Kv7 channels"],"prefix":"10.1152","volume":"330","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2337-2312","authenticated-orcid":false,"given":"Srijit","family":"Ghosh","sequence":"first","affiliation":[{"name":"Dundalk Institute of Technology","place":["Ireland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaneez E.","family":"Rabab","sequence":"additional","affiliation":[{"name":"Dundalk Institute of Technology","place":["Ireland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Hollywood","sequence":"additional","affiliation":[{"name":"Dundalk Institute of Technology","place":["Ireland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0750-0847","authenticated-orcid":false,"given":"Keith D.","family":"Thornbury","sequence":"additional","affiliation":[{"name":"Dundalk Institute of Technology","place":["Ireland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9443-5491","authenticated-orcid":false,"given":"Gerard P.","family":"Sergeant","sequence":"additional","affiliation":[{"name":"Dundalk Institute of Technology","place":["Ireland"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198408163110701"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/s0163-7258(03)00004-4"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/s0024-3205(01)01053-0"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1703501"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqab053"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26125455"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00188.2024"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00277"},{"key":"B9","first-page":"21","volume-title":"Calcium Signaling in Airway Smooth Muscle Cells","author":"Byron KL","year":"2013"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2013.12.003"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00253.2013"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-2-00349.x"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200910313"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2597-05.2005"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14910"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00980.2024"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1007\/s004240050427"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.07.060"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.05.002"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1124\/mol.114.093799"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1016\/s0898-6568(99)00035-2"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.044750"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.527820"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.303405"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.433383"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805216200"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00195"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.12.003"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305167110"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1126\/science.1131163"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1785"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1016\/s0024-3205(97)00433-5"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1124\/mol.115.101758"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19082223"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/s0165-6147(00)01775-2"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00167.2014"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqac050"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2004.11.009"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2016.10.023"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-010617-052912"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1016\/s0896-6273(02)00790-0"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.23-12-04931.2003"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000118248.17466.B7"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74062-9"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)50057-0"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98401-2"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.06.013"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.198200"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2011-0088OC"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.70.1.281"},{"key":"B51","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2020.10.027"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1418605112"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1124\/mol.119.117192"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1021\/ml200053x"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.115.225268"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1113\/JP272762"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.01004"},{"key":"B58","volume-title":"Probe Reports from the NIH Molecular Libraries Program","author":"Yu H","year":"2013"}],"container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.00752.2025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T19:26:46Z","timestamp":1776972406000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.00752.2025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,1]]},"references-count":58,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4,1]]}},"alternative-id":["10.1152\/ajpcell.00752.2025"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.00752.2025","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,1]]},"assertion":[{"value":"2025-10-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-04","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}