{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:08:02Z","timestamp":1718669282693},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1979,9,1]]},"abstract":"<jats:p> In primary cultures of chicken skeletal muscle, decreasing the [CO2] of the gaseous phase below 10(-3)% resulted in inhibition of cell proliferation and cytolysis. With 10(-3)% CO2-air, cell proliferation was slightly retarded and myotube formation was inhibited approximately 90% compared to cultures receiving 5% CO2-air. Changes in pH were not effective. Culture in low [CO2] resulted in the accumulation of lipoidal inclusions and unique cytoplasmic structures. Increasing time in culture with low [CO2] resulted in an increase in the length of G1 of the cell cycle. The inhibition was reversed by the addition of 5% CO2-air at any time in culture up to 2 wk with a minimum time of 3--6 h required. Lipoidal inclusions decreased in number and the unique cytoplasmic structures were absent. During the first 3 days in culture, myoblasts showing dependence on [CO2] for myotube formation increased in number, and the effect of elevated [CO2] on these cells was long lasting. The data suggest that some aspect of myoblast differentiation relating to cell recognition and fusion is affected by decreased [CO2]. <\/jats:p>","DOI":"10.1152\/ajpcell.1979.237.3.c166","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T10:40:44Z","timestamp":1514112044000},"page":"C166-C176","source":"Crossref","is-referenced-by-count":4,"title":["Reversible suppression of skeletal myotube formation in vitro obtained by varying [CO2]"],"prefix":"10.1152","volume":"237","author":[{"given":"R. J.","family":"Przybylski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Bullaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"MacBride","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1979.237.3.C166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:57:06Z","timestamp":1718654226000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1979.237.3.C166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1979,9,1]]}},"alternative-id":["10.1152\/ajpcell.1979.237.3.C166"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1979.237.3.c166","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,9,1]]}}}