{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:06:30Z","timestamp":1718669190021},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1984,1,1]]},"abstract":"<jats:p> Insulin release, Ca2+ fluxes, and adenosine 3',5'-cyclic monophosphate (cAMP) levels were measured under various conditions to test the selectivity of cobalt (Co2+) actions in pancreatic islets. Insulin release was measured simultaneously either with 45Ca2+ uptake or 45Ca2+ efflux from rat islets maintained 2 days in tissue culture. Glucose (16.7 mM)-stimulated insulin release was inhibited by Co2+ (0.25, 0.5 mM) without concomitant inhibition of glucose-stimulated 45Ca2+ uptake. At higher concentrations of Co2+ (1.0, 2.5 mM) glucose- as well as K+-stimulated insulin release were inhibited in parallel with 45Ca2+ uptake. In the presence or the absence of external Ca2+, Co2+ (0.5 mM) also decreased 3-isobutyl-1-methylxanthine-stimulated insulin release, which does not depend on the stimulation of Ca2+ uptake. Co2+ did not alter islet cAMP levels. Co2+ also did not affect the unidirectional basal 45Ca2+ efflux measured in the absence of Ca2+ but completely overcame the stimulated 45Ca2+ efflux evoked by the removal of external Mg2+. Under the latter conditions, 50, but not 5 microM verapamil, decreased the stimulated 45Ca2+ efflux. It is concluded that Co2+ is able to inhibit insulin release and lower Ca2+ efflux without apparent interference with Ca2+ uptake. This suggests that Co2+, in addition to its well-known inhibition of Ca2+ uptake, may exert its action at a distal step in the insulin release process. <\/jats:p>","DOI":"10.1152\/ajpcell.1984.246.1.c57","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T11:46:51Z","timestamp":1514116011000},"page":"C57-C62","source":"Crossref","is-referenced-by-count":8,"title":["Cobalt inhibition of insulin release: evidence for an action not related to Ca2+ uptake"],"prefix":"10.1152","volume":"246","author":[{"given":"C. B.","family":"Wollheim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Janjic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1984.246.1.C57","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:54:27Z","timestamp":1718654067000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1984.246.1.C57"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,1,1]]}},"alternative-id":["10.1152\/ajpcell.1984.246.1.C57"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1984.246.1.c57","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,1,1]]}}}