{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:33:24Z","timestamp":1762076004606},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1986,2,1]]},"abstract":"<jats:p> The effects on insulin release of Na+ removal, alteration of extracellular pH, and inhibition of acid extrusion processes were examined using freshly isolated and 46-h cultured islets of Langerhans. Na+ removal inhibited the secretory response to 16.7 mM glucose specifically in fresh islets but stimulated the low-glucose response in cultured islets. These divergent effects of Na+ depletion were perfectly mimicked by amiloride, an inhibitor of Na+-H+ exchange, and could be overcome in a raised HCO-3, pH 8.0 medium. Simply raising extracellular pH at constant HCO-3 had no effect. Na+ removal also inhibited the secretory responses to other nutrient secretagogues such as D-glyceraldehyde and L-leucine but not to agents like 3-isobutyl-1-methylxanthine or 12-O-tetradecanoylphorbol-13-acetate. The results show that Na+ is not itself essential for the secretory process but rather suggest that it plays a permissive role in the regulation of intracellular pH via Na+-H+ exchange. Such a regulation appears important mainly for nutrient-stimulated insulin release, which is associated with the generation of acidic metabolites. <\/jats:p>","DOI":"10.1152\/ajpcell.1986.250.2.c207","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:08:22Z","timestamp":1514106502000},"page":"C207-C213","source":"Crossref","is-referenced-by-count":22,"title":["Sodium requirement for insulin release: putative role in regulation of intracellular pH"],"prefix":"10.1152","volume":"250","author":[{"given":"T. J.","family":"Biden","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Janjic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. B.","family":"Wollheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1986.250.2.C207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:53:04Z","timestamp":1718653984000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1986.250.2.C207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,2,1]]}},"alternative-id":["10.1152\/ajpcell.1986.250.2.C207"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1986.250.2.c207","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,2,1]]}}}