{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:32:34Z","timestamp":1760646754425},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1988,10,1]]},"abstract":"<jats:p> 42K+ transport properties of isolated rat parotid acini were characterized concomitant with measurements of membrane potentials (Em) by means of the fluorescent dye diSC3-(5). In unstimulated acini suspended in a 5 mM K+ buffer, Em was governed by the K+ and Cl- gradients and amounted to about -59 mV, a value that remained unaffected on cholinergic stimulation. In unstimulated acini, 42K+ influx was largely mediated by the Na+-K+ pump, and the residual influxes were mediated by a bumetanide-sensitive component (cotransport system) and by K+ channels. Efflux of 42K+ was largely mediated by a bumetanide-sensitive component and by K+ channels. In the unstimulated state, the cotransport system was mediating K+-K+ exchange without contributing to the net uptake of K+. Within 10 s after stimulation, a approximately 10-fold increase in the acinar K+ conductance (gK) occurred, resulting in a rapid net efflux of K+ that amounted to approximately 3.8 mmol.l cells-1.s-1. Measurements of 42K+ fluxes as a function of the external K+ concentration revealed that in the stimulated state gK increases when external K+ is raised from 0.7 to 10 mM, consistent with an activation of acinar gK by the binding of external K+ to the channel. 42K+ flux ratios as well as the effect of the K+ channel inhibitor from scorpion venom (LQV) suggest that approximately 90% of K+ transport in the stimulated state is mediated by \"maxi\" K+ channels. <\/jats:p>","DOI":"10.1152\/ajpcell.1988.255.4.c508","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T09:58:29Z","timestamp":1514109509000},"page":"C508-C518","source":"Crossref","is-referenced-by-count":21,"title":["K+ transport and membrane potentials in isolated rat parotid acini"],"prefix":"10.1152","volume":"255","author":[{"given":"B.","family":"Nauntofte","sequence":"first","affiliation":[{"name":"Department of Oral Function and Physiology, Royal Dental College,University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Dissing","sequence":"additional","affiliation":[{"name":"Department of Oral Function and Physiology, Royal Dental College,University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1988.255.4.C508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:50:14Z","timestamp":1718653814000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1988.255.4.C508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988,10,1]]}},"alternative-id":["10.1152\/ajpcell.1988.255.4.C508"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1988.255.4.c508","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,10,1]]}}}