{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:39:28Z","timestamp":1791351568280,"version":"4.3.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1989,1,1]]},"abstract":"<jats:p> The purpose of this study was to compare the effects of atriopeptin III (AP-III), vasoactive intestinal peptide (VIP), and ionomycin on Na and Cl influx and to correlate changes in transport with effects on intracellular adenosine 3',5'-cyclic monophosphate (cAMP) and guanosine 5'-cyclic monophosphate (cGMP) content of the tissue. In addition, the question of whether AP-III inhibits ion transport directly by acting on enterocyte receptors for AP-III or indirectly by stimulation of enteric nerves in the submucosa was also addressed. The results showed that AP-III, ionomycin, and bumetanide all inhibited the initial rate of Na and Cl influx, suggesting that they directly block Na-K-2Cl cotransport activity. VIP had no effect on unidirectional influx of Na and Cl. AP-III caused a fourfold increase in intracellular [cGMP] without any significant effect on [cAMP]. VIP stimulated [cAMP] by fourfold but had no effect on [cAMP]. Ionomycin had no effect on either [cAMP] or [cGMP]. Inhibition of transport by AP-III could not be blocked by tetrodotoxin (TTX), indicating that enteric nerves in the submucosa are not directly involved in mediating the effects of AP-III on epithelial ion transport. The observation that two classes of neuronal depolarizing agents (veratrine and scorpion venom) cause TTX-sensitive inhibition of basal ion transport establishes that NaCl absorption in flounder intestine is subject to regulation by enteric nerves located in the submucosa. <\/jats:p>","DOI":"10.1152\/ajpcell.1989.256.1.c142","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:12:28Z","timestamp":1514128348000},"page":"C142-C146","source":"Crossref","is-referenced-by-count":113,"title":["Cyclic nucleotide-mediated effects of ANF and VIP on flounder intestinal ion transport"],"prefix":"10.1152","volume":"256","author":[{"given":"S. M.","family":"O'grady","sequence":"first","affiliation":[{"name":"Department of Veterinary Biology, University of Minnesota, St. Paul55108."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1989.256.1.C142","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:50:11Z","timestamp":1718653811000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1989.256.1.C142"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,1,1]]}},"alternative-id":["10.1152\/ajpcell.1989.256.1.C142"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1989.256.1.c142","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,1,1]]}}}