{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:43:35Z","timestamp":1761709415710},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1989,10,1]]},"abstract":"<jats:p> Cytoplasmic pH (pHi) was monitored using the pH-sensitive absorbance of 5(6)carboxy-4',5'-dimethylfluorescein in monolayers of a cell clone derived from bovine pigmented ciliary epithelium (PE) transformed with the simian virus 40. 1) Changing extracellular media from a nominally HCO3(-)-free solution to a solution containing 28 mM HCO3(-)-5% CO2 at constant extracellular pH (7.4) resulted in a delayed alkalinization of pHi, which was 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) sensitive and was inhibited in Na+-free medium and in Cl(-)-depleted cells. 2) DIDS pretreatment acidified pHi in HCO3(-)-containing media. 3) Replacing extracellular Cl- resulted in a DIDS-sensitive, HCO3(-)-dependent, and Na+-independent alkalinization. 4) Replacing extracellular Na+ in HCO3(-)-containing media led to a partly DIDS-sensitive intracellular acidification. 5) Recovery of pHi after an alkali load (acetate prepulse) had a HCO3(-)-dependent and DIDS-sensitive component. 6) Two Na+-dependent components participated in pHi regulation after an acid load (NH4+ prepulse) in HCO3(-)-containing solution. One was amiloride sensitive, the other was DIDS sensitive and was inhibited in HCO3(-)-free media and after Cl- depletion. We conclude that in cultured PE, in addition to Na+-H+ exchange, two HCO3-transporters participate in pHi regulation. Cl(-)-dependent Na+-HCO3-symport regulates pHi during steady state and after an acid load, and Na+-independent Cl(-)-HCO3-exchange is involved in pHi recovery after an alkali load. <\/jats:p>","DOI":"10.1152\/ajpcell.1989.257.4.c696","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:26:16Z","timestamp":1514129176000},"page":"C696-C705","source":"Crossref","is-referenced-by-count":17,"title":["Role of HCO3- in regulation of cytoplasmic pH in ciliary epithelial cells"],"prefix":"10.1152","volume":"257","author":[{"given":"H.","family":"Helbig","sequence":"first","affiliation":[{"name":"Institut fur Klinische Physiologie, Klinikum Steglitz der FreienUniversitat Berlin, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Korbmacher","sequence":"additional","affiliation":[{"name":"Institut fur Klinische Physiologie, Klinikum Steglitz der FreienUniversitat Berlin, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Stumpff","sequence":"additional","affiliation":[{"name":"Institut fur Klinische Physiologie, Klinikum Steglitz der FreienUniversitat Berlin, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Coca-Prados","sequence":"additional","affiliation":[{"name":"Institut fur Klinische Physiologie, Klinikum Steglitz der FreienUniversitat Berlin, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Wiederholt","sequence":"additional","affiliation":[{"name":"Institut fur Klinische Physiologie, Klinikum Steglitz der FreienUniversitat Berlin, Federal Republic of Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1989.257.4.C696","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:48:42Z","timestamp":1718653722000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1989.257.4.C696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,10,1]]}},"alternative-id":["10.1152\/ajpcell.1989.257.4.C696"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1989.257.4.c696","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,1]]}}}