{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:24:22Z","timestamp":1760171062866},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1989,10,1]]},"abstract":"<jats:p> Voltage-activated ion currents were measured in cultured skeletal muscle myoballs. Cultures were generated from biopsies from patients referred for diagnosis of susceptibility to malignant hyperthermia (MH); diagnosis of susceptibility (MH+) or nonsusceptibility (MH-) was made on the basis of in vitro halothane-induced contracture of a separate piece of biopsy. Measurements of ion currents were made at room temperature in the absence of anesthetic agents, using tight-seal whole-cell recording. Fast transient Na+ currents and delayed outward K+ currents were similar in magnitude and kinetics in cells from MH+ and MH- patients. An additional slowly inactivating inward current component was commonly observed in cells from MH+ patients. This current was blockable by tetrodotoxin and was carried by Na+ but not by Ba2+. The component was less frequently observed and was of a lower magnitude in cells from MH- patients. The increased magnitude of the slow inward current observed in cultured muscle cells from MH+ patients may be a manifestation of the lesion that causes MH. <\/jats:p>","DOI":"10.1152\/ajpcell.1989.257.4.c759","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:26:16Z","timestamp":1514129176000},"page":"C759-C765","source":"Crossref","is-referenced-by-count":25,"title":["Malignant hyperthermia: slow sodium current in cultured human muscle cells"],"prefix":"10.1152","volume":"257","author":[{"given":"S. J.","family":"Wieland","sequence":"first","affiliation":[{"name":"Department of Anatomy, Hahnemann University, Philadelphia,Pennsylvania 19102."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Fletcher","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Hahnemann University, Philadelphia,Pennsylvania 19102."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Rosenberg","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Hahnemann University, Philadelphia,Pennsylvania 19102."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q. H.","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Hahnemann University, Philadelphia,Pennsylvania 19102."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1989.257.4.C759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:48:31Z","timestamp":1718653711000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1989.257.4.C759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,10,1]]}},"alternative-id":["10.1152\/ajpcell.1989.257.4.C759"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1989.257.4.c759","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,1]]}}}