{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T20:49:41Z","timestamp":1771879781354,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1989,11,1]]},"abstract":"<jats:p> The properties of Ca2+ channels in cultured rat pituitary gonadotrophs were analyzed by the patch-clamp technique. The inward Ca2+ currents, recorded in the presence of 5.2 mM Ca2+ or Ba2+, included a fast, transient component with activation-inactivation kinetics and a delayed component with slower activation. The midpoint of the activation curve lay at -30 mV for the transient component and at -12 mV for the delayed component. At the midpoint, changes in potential of 9.5 and 13 mV induced an e-fold change in the activation of the transient and delayed components, respectively. The rate of inactivation of the first component was strongly voltage dependent. At -43 mV, a 7.4-mV change in potential induced an e-fold change in the fraction of Ca2+ channels available to conduct Ca2+ current. During long-lasting (100-200 ms) low-frequency depolarizing voltage-clamp pulses, the size of the delayed component of the Ca2+ current remained constant. The differential effects of membrane potential on inactivation and the different time constants for activation of the two components of the Ca2+ conductance indicate the presence of two types of Ca2+ channels in the membrane of the gonadotroph: the rapidly inactivating current appears to be attributable to a T-type channel, and the noninactivating current corresponds to the L-type channel described in many other cell types. <\/jats:p>","DOI":"10.1152\/ajpcell.1989.257.5.c865","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:21:53Z","timestamp":1514128913000},"page":"C865-C874","source":"Crossref","is-referenced-by-count":49,"title":["Characteristics of two types of calcium channels in rat pituitary gonadotrophs"],"prefix":"10.1152","volume":"257","author":[{"given":"A.","family":"Stutzin","sequence":"first","affiliation":[{"name":"Laboratory of Cell Biology and Genetics, National Institute ofDiabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. S.","family":"Stojilkovic","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology and Genetics, National Institute ofDiabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. J.","family":"Catt","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology and Genetics, National Institute ofDiabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Rojas","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology and Genetics, National Institute ofDiabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1989.257.5.C865","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:48:15Z","timestamp":1718653695000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1989.257.5.C865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1989,11,1]]}},"alternative-id":["10.1152\/ajpcell.1989.257.5.C865"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1989.257.5.c865","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,11,1]]}}}