{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:15:18Z","timestamp":1762161318324},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1990,3,1]]},"abstract":"<jats:p> The concentration of ionized cytosolic calcium [( Ca2+]i) was determined in peripheral blood mononuclear cells from normal and malignant hyperthermia (MH)-susceptible humans and pigs, using the fluorescent Ca2+ indicator indo-1. [Ca2+]i was slightly but significantly elevated in cells from MH human cells relative to normal cells (198 +\/- 18 nM, n = 15, and 146 +\/- 14 nM, n = 11, respectively, P less than 0.05). Anesthetic concentrations of halothane in the cell suspension resulted in a rapid increase in [Ca2+]i in cells from both normal and MH humans or pigs. The increases (delta) were more pronounced in cells from MH subjects than from normal individuals (delta at 5.7 mM halothane: 245 +\/- 53 vs. 57 +\/- 11 nM, respectively) and from MH than from normal pigs (delta of 241 +\/- 63 vs. 53 +\/- 27 nM, respectively). Removal of extracellular Ca2+ obliterated the delta[Ca2+]i caused by halothane in cells from normal humans or pigs but only decreased by about half the delta[Ca2+]i in cells from MH humans or pigs. In 1,2-bis-(aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-loaded cells, in the absence of extracellular Ca2+, halothane failed to increase [Ca2+]i. This suggests that buffering Cai2+ with BAPTA precludes detection of release of Ca2+ from intracellular stores, explaining the previous observations made with quin2, a highly chelating Ca2+ indicator. It is concluded that clinical concentrations of halothane allow influx of Ca2+ in cells from both normal and MH-susceptible individuals but release Ca2+ from intracellular stores selectively in cells from the latter group.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1990.258.3.c495","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:37:40Z","timestamp":1514129860000},"page":"C495-C503","source":"Crossref","is-referenced-by-count":18,"title":["Halothane-dependent release of intracellular Ca2+ in blood cells in malignant hyperthermia"],"prefix":"10.1152","volume":"258","author":[{"given":"A.","family":"Klip","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Hospital for Sick Children, Toronto,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. B.","family":"Mills","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Hospital for Sick Children, Toronto,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. A.","family":"Britt","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Hospital for Sick Children, Toronto,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. E.","family":"Elliott","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Hospital for Sick Children, Toronto,Ontario, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1990.258.3.C495","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:47:21Z","timestamp":1718653641000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1990.258.3.C495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,3,1]]}},"alternative-id":["10.1152\/ajpcell.1990.258.3.C495"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1990.258.3.c495","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3,1]]}}}