{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:56:35Z","timestamp":1771642595336,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1990,4,1]]},"abstract":"<jats:p> The kinetics of Na(+)-K(+)-Cl- cotransport were studied by measuring ouabain-insensitive furosemide-sensitive Rb+ influx (JRb) into HeLa cells while varying the cellular ATP and the extracellular Rb+ and Na+ concentrations. Results reveal that ATP stimulates JRb by increasing the affinity of the cotransporter for Rb+ (K+), and the apparent Michaelis constant (Km) for ATP was 0.95 +\/- 0.03 mmol\/l cell water. Two ATP molecules may relate to the uptake of one Rb+ by the cotransport pathway, as examined by the nonlinear least-squares method for goodness-of-fit and a Hill plot, JRb was strengthened by an increase in the inward chemical gradient associated with cell swelling on preincubation in a low-Na+ high-K+ medium, accompanying an increase in the affinity of the transporter for ATP. JRb was apparently activated by extracellular Na+, and the activation was enhanced by an increase in the cellular ATP concentration. Lactate production stimulated by 2 microM carbonylcyanide m-chlorophenyl hydrazone (CCCP) was reduced by 10 microM ouabain but not altered by further addition of 0.1 mM furosemide. Increases in cellular adenosine 3',5'-cyclic monophosphate (cAMP) caused by treatment with 0.1 mM isoproterenol plus 0.5 mM 3-isobutyl-1-methylxanthine or with 0.1 mM dibutyryl cAMP did not influence JRb. From this and previous studies, we propose a general and a specific model of Na(+)-K(+)-Cl- cotransport, which elucidate the order of binding of extracellular ions and reaction of cellular ATP. <\/jats:p>","DOI":"10.1152\/ajpcell.1990.258.4.c599","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:32:04Z","timestamp":1514129524000},"page":"C599-C609","source":"Crossref","is-referenced-by-count":18,"title":["Kinetic mechanism of ATP action in Na(+)-K(+)-Cl- cotransport of HeLa cells determined by Rb+ influx studies"],"prefix":"10.1152","volume":"258","author":[{"given":"T.","family":"Ikehara","sequence":"first","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Tokushima,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Tokushima,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Tokushima,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Medicine, University of Tokushima,Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1990.258.4.C599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:47:11Z","timestamp":1718653631000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1990.258.4.C599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1990,4,1]]}},"alternative-id":["10.1152\/ajpcell.1990.258.4.C599"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1990.258.4.c599","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,4,1]]}}}