{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T17:10:17Z","timestamp":1773249017554,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:p> Human platelets can be stimulated by recombinant human fifth component of complement (rhC5a) in the presence of human neutrophils. After challenge with N-formyl-Met-Leu-Phe or rhC5a, concentrated neutrophils release cathepsin G into the supernatant. The concentrations of cathepsin G recovered by titration of the enzymatic activity correlate with the capability of these supernatants to induce platelet stimulation as measured by serotonin release. Cathepsin G purified from neutrophil granules triggered platelet aggregation and serotonin release independent of arachidonic acid metabolites and platelet-activating factor formation. A concentration of 100 nM of cathepsin G, which was reached in the surrounding space of activated neutrophils, induced a 50% platelet stimulation. Three distinct antiproteinases were tested against cathepsin G-induced platelet activation. Z-Gly-Leu-Phe-CH2Cl, a specific inhibitor of cathepsin G enzymatic activity, proved to be nonspecific in our biological system. By contrast, alpha 1-antichymotrypsin and alpha 1-antitrypsin displayed specific activities. The physiological specific inhibitor of cathepsin G, alpha 1-antichymotrypsin, was the most potent and was used in the rhC5a-induced neutrophils-mediated platelet activation. A complete inhibition was achieved, showing that release of cathepsin G from neutrophils accounts for platelet activation. Such a chain of events involving C5a, neutrophils, cathepsin G, and platelets may be of relevance in certain inflammatory states, particularly the adult respiratory distress syndrome. <\/jats:p>","DOI":"10.1152\/ajpcell.1990.258.6.c1100","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:56:27Z","timestamp":1514130987000},"page":"C1100-C1107","source":"Crossref","is-referenced-by-count":56,"title":["Activation of human platelets by C5a-stimulated neutrophils: a role for cathepsin G"],"prefix":"10.1152","volume":"258","author":[{"given":"P.","family":"Ferrer-Lopez","sequence":"first","affiliation":[{"name":"Unite Associee IP-Institut National de la Sante et de la Recherche,Medicale 285, Institut Pasteur, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Renesto","sequence":"additional","affiliation":[{"name":"Unite Associee IP-Institut National de la Sante et de la Recherche,Medicale 285, Institut Pasteur, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Schattner","sequence":"additional","affiliation":[{"name":"Unite Associee IP-Institut National de la Sante et de la Recherche,Medicale 285, Institut Pasteur, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Bassot","sequence":"additional","affiliation":[{"name":"Unite Associee IP-Institut National de la Sante et de la Recherche,Medicale 285, Institut Pasteur, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Laurent","sequence":"additional","affiliation":[{"name":"Unite Associee IP-Institut National de la Sante et de la Recherche,Medicale 285, Institut Pasteur, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Chignard","sequence":"additional","affiliation":[{"name":"Unite Associee IP-Institut National de la Sante et de la Recherche,Medicale 285, Institut Pasteur, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1990.258.6.C1100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:47:43Z","timestamp":1718653663000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1990.258.6.C1100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,6,1]]}},"alternative-id":["10.1152\/ajpcell.1990.258.6.C1100"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1990.258.6.c1100","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,1]]}}}