{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:30:07Z","timestamp":1790811007254,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1990,8,1]]},"abstract":"<jats:p> We developed a convenient flux assay that permits simultaneous measurement of Cl and K conductance pathways in Cl-secreting epithelial cells. Monolayers of the colonic tumor cell line T84 were preloaded with 125I and 86Rb, and isotope effluxes were monitored by a sample-replace procedure. The adenosine 3',5'-cyclic monophosphate (cAMP)-mediated agonists forskolin and prostaglandin E2 increased I efflux with little effect on Rb efflux, whereas the Ca-mediated agonists ionomycin, A23187, and carbachol increased both I and Rb effluxes. Simultaneous determinations of I and Cl or Rb and K effluxes indicated that I and Rb provide good measures of the effluxes of Cl and K, respectively. Forskolin- and ionomycin-stimulated I effluxes were inhibited by the Cl-channel blockers diphenylamine-2-dicarboxylate (DPC), 5-nitro-2-(3-phenylpropyl-amino)benzoic acid (NPPB), and 2-[cyclopentyl-6,7-dichloro-2,3-dihydro-2-methyl-1-oxo-1H- inden-5-yl)oxy]acetic acid (IAA-94) and by high external K. The Rb efflux evoked by ionomycin was inhibited by the K-channel blockers Ba and charybdotoxin. These findings suggest that I and Rb effluxes provide qualitative estimates of agonist-stimulated Cl and K conductance pathways. Thus this method can provide a simple and relatively inexpensive screening assay for Cl and K conductances in cultured cells to assess the effects of agonist, blockers, or genetic manipulations. <\/jats:p>","DOI":"10.1152\/ajpcell.1990.259.2.c358","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:53:12Z","timestamp":1514130792000},"page":"C358-C364","source":"Crossref","is-referenced-by-count":132,"title":["A simple assay for agonist-regulated Cl and K conductances in salt-secreting epithelial cells"],"prefix":"10.1152","volume":"259","author":[{"given":"C. J.","family":"Venglarik","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of Alabama,Birmingham 35294."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Bridges","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Alabama,Birmingham 35294."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. A.","family":"Frizzell","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of Alabama,Birmingham 35294."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1990.259.2.C358","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:47:56Z","timestamp":1718653676000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1990.259.2.C358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,8,1]]}},"alternative-id":["10.1152\/ajpcell.1990.259.2.C358"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1990.259.2.c358","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8,1]]}}}