{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:07:15Z","timestamp":1718669235289},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1990,12,1]]},"abstract":"<jats:p> The effect of vasoactive intestinal peptide (VIP) and forskolin on carbachol-induced K+ release from superfused rat submandibular and parotid gland fragments was examined using a K(+)-sensitive electrode. Carbachol (0.1, 1, and 10 microM) superfused over the glandular fragments for 15 min caused a concentration-dependent, transient elevation of K+ efflux, with a peak value after approximately 5 min. The carbachol-induced release of K+ could be divided into two distinct components, one transient peak lasting 5-8 min independent of extracellular Ca2+ and a second component of K+ release dependent on Ca2+ in the perfusion medium. VIP (1 microM) lacked effect on K+ efflux on its own but increased the carbachol (1 microM)-evoked K+ release. The VIP effects on K+ efflux were mimicked by forskolin (10 microM). Omission of Ca2+ from the medium totally abolished the augmenting effect of VIP and forskolin on carbachol-evoked K+ efflux. The Ca2+ ionophore A23187 (1 or 10 microM) induced a prolonged low-rate efflux of K+, which was dependent on Ca2+ in the medium. This effect of A23187 on K+ secretion was potentiated by forskolin (10 microM). The Na(+)-K(+)-ATPase blocker ouabain did not affect K+ release on its own, a lack of effect which remained following pretreatment with forskolin. It is concluded that VIP, by increasing the intracellular levels of cAMP in the glandular cell, potentiates carbachol-evoked Ca2(+)-dependent K+ efflux. These results may help to explain the synergistic effects of the coexisting transmitters VIP and acetylcholine. <\/jats:p>","DOI":"10.1152\/ajpcell.1990.259.6.c904","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:54:03Z","timestamp":1514120043000},"page":"C904-C910","source":"Crossref","is-referenced-by-count":16,"title":["VIP and forskolin enhance carbachol-induced K+ efflux from rat salivary gland fragments by a Ca2(+)-sensitive mechanism"],"prefix":"10.1152","volume":"259","author":[{"given":"O.","family":"Larsson","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Detsch","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. B.","family":"Fredholm","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1990.259.6.C904","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:46:59Z","timestamp":1718653619000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1990.259.6.C904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,12,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,12,1]]}},"alternative-id":["10.1152\/ajpcell.1990.259.6.C904"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1990.259.6.c904","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,12,1]]}}}