{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:37:15Z","timestamp":1772897835919,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1991,2,1]]},"abstract":"<jats:p> The effects of 8-bromoguanosine 3',5'-cyclic monophosphate (8-BrcGMP) on apical membrane cation conductances in the toad urinary bladder were investigated. 8-BrcGMP (1 mM) added to the serosal solution increased the amiloride-sensitive short-circuit current (INa) after a delay of 5 min to a steady-state value 1.8 times that of controls achieved after 30 min. Similar effects were seen when the bladders were bathed on the serosal side with a normal NaCl Ringer solution and with a high-K sucrose solution to depolarize the basolateral membrane. Under the latter conditions, the amiloride-sensitive transepithelial conductance increased in parallel with the short-circuit current, indicating stimulation of apical membrane Na channels. The threshold concentration for observing the stimulation of INa was 100 microM, 10-100 times larger than the concentration of 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) required to elicit an increase in INa. Currents through an outwardly rectifying Ca-sensitive cation conductance (Iout) were also increased by 1.8-fold relative to controls. This stimulatory effect occurred after a delay of 15 min and reached maximal levels 90-120 min after addition of the nucleotide. The effects of cGMP on INa were not additive with those of 8-BrcAMP or with antidiuretic hormone, an agent known to act by increasing cAMP within the cell. Addition of 1 mM 3-isobutyl-1-methylxanthine to the serosal side of the bladders stimulated INa by 1.3-fold and Iout by 2.4-fold. In both cases, subsequent addition of cGMP produced no further activation of either conductance.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1991.260.2.c234","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:52:38Z","timestamp":1514119958000},"page":"C234-C241","source":"Crossref","is-referenced-by-count":33,"title":["Stimulation by cGMP of apical Na channels and cation channels in toad urinary bladder"],"prefix":"10.1152","volume":"260","author":[{"given":"S.","family":"Das","sequence":"first","affiliation":[{"name":"Department of Physiology, Cornell University Medical College, NewYork, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Garepapaghi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cornell University Medical College, NewYork, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. G.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Physiology, Cornell University Medical College, NewYork, New York 10021."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1991.260.2.C234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:59Z","timestamp":1718653499000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1991.260.2.C234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,2,1]]}},"alternative-id":["10.1152\/ajpcell.1991.260.2.C234"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1991.260.2.c234","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2,1]]}}}