{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T18:37:42Z","timestamp":1784227062509,"version":"3.55.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1991,5,1]]},"abstract":"<jats:p> We measured free intracellular Ca2+ concentration ([Ca2+]i) and Ca2+ current (ICa) simultaneously in voltage-clamped, indo-1-loaded smooth muscle cells isolated from the circular layer of the canine antrum. Resting [Ca2+]i averaged 144 +\/- 20 nM in cells held at -70 mV. Depolarization positive to -50 mV elicited ICa and increased [Ca2+]i. Peak [Ca2+]i occurred between 0 and +10 mV and averaged 372 +\/- 48 nM. On repolarization, [Ca2+]i decreased slowly (time constant 2-3 s) and the rate depended on the magnitude of [Ca2+]i. Cells were also voltage clamped with protocols that mimicked the upstroke and plateau phases of slow waves. With simulated plateau potentials of -55 to -45 mV, [Ca2+]i increased transiently as a result of the small transient ICa elicited by the upstroke depolarization. Sustained ICa was of sufficient magnitude with plateau depolarizations positive to -40 mV to cause a secondary rise in [Ca2+]i throughout the plateau phase. These data suggest that at the plateau potential of slow waves in situ, ICa is sufficient to cause a sustained increase in [Ca2+]i. The resulting accumulation of Ca2+ may couple the slow wave plateau to contraction and may increase the open probability of Ca(2+)-activated K channels. The latter may provide the outward current necessary to initiate repolarization. <\/jats:p>","DOI":"10.1152\/ajpcell.1991.260.5.c1012","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:30:49Z","timestamp":1514118649000},"page":"C1012-C1018","source":"Crossref","is-referenced-by-count":49,"title":["Relationship between calcium current and cytosolic calcium in canine gastric smooth muscle cells"],"prefix":"10.1152","volume":"260","author":[{"given":"F.","family":"Vogalis","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Nevada School of Medicine,Reno 89511."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. G.","family":"Publicover","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Nevada School of Medicine,Reno 89511."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"Hume","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Nevada School of Medicine,Reno 89511."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. M.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Nevada School of Medicine,Reno 89511."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1991.260.5.C1012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:46:36Z","timestamp":1718653596000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1991.260.5.C1012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,5,1]]}},"alternative-id":["10.1152\/ajpcell.1991.260.5.C1012"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1991.260.5.c1012","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5,1]]}}}