{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:40:07Z","timestamp":1791092407312,"version":"4.1.0"},"reference-count":0,"publisher":"American Physiological Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1991,7,1]]},"abstract":"<jats:p> Increased levels of corticosteroids result in the development of hypertension in vivo. To investigate whether corticosteroids modulate calcium handling in vascular smooth muscle cells, we studied 45Ca2+ uptake and binding of [methyl-3H]PN 200-110, a potent dihydropyridine Ca2+ antagonist, in A7r5 vascular smooth muscle cells. Forty-eight-hour treatment with 100 nM dexamethasone increased the unidirectional 45Ca2+ uptake during a 2-min period, and the 30-min 45Ca2+ uptake of dexamethasone-treated cells was 95% greater than that of nontreated cells. The lag time for the dexamethasone effect on Ca2+ uptake was approximately 8 h. The effect of dexamethasone was blocked by the glucocorticoid antagonist RU 38486, whereas it was not affected by the mineralocorticoid antagonist RU 26752. After cessation of the dexamethasone treatment, 45Ca2+ uptake returned to the control level by 24 h. The effect of dexamethasone was completely blocked by nifedipine in a dose-dependent manner. Scatchard plots of [methyl-3H]PN 200-110 binding revealed two binding sites (Kd; 0.02 and 1 nM), and dexamethasone increased the number of the higher affinity binding sites. These results indicate that glucocorticoids increase Ca2+ uptake possibly mediated by an increase in the number of dihydropyridine-sensitive Ca2+ channels. <\/jats:p>","DOI":"10.1152\/ajpcell.1991.261.1.c106","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:40:39Z","timestamp":1514119239000},"page":"C106-C114","source":"Crossref","is-referenced-by-count":80,"title":["Glucocorticoids increase Ca2+ uptake and [3H]dihydropyridine binding in A7r5 vascular smooth muscle cells"],"prefix":"10.1152","volume":"261","author":[{"given":"T.","family":"Hayashi","sequence":"first","affiliation":[{"name":"Third Department of Internal Medicine, Fukui Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Nakai","sequence":"additional","affiliation":[{"name":"Third Department of Internal Medicine, Fukui Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Miyabo","sequence":"additional","affiliation":[{"name":"Third Department of Internal Medicine, Fukui Medical School, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1991.261.1.C106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:45:58Z","timestamp":1718653558000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1991.261.1.C106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,7,1]]}},"alternative-id":["10.1152\/ajpcell.1991.261.1.C106"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1991.261.1.c106","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7,1]]}}}