{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:48:51Z","timestamp":1760204931009},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1991,11,1]]},"abstract":"<jats:p> Organotin derivatives represent a class of artificial ionophores that mediate Cl(-)-OH- exchange and thereby facilitate the chemical equilibrium distribution of Cl- and H+ across biological membranes. Imposing different pH and Cl- gradients by varying extracellular pH (pHo) and extracellular [Cl-] in the presence of 1 microM tributyltin validated the above assumptions in human neutrophils. Under relatively alkaline conditions [intracellular pH (pHi) greater than or equal to 7.10 and pHo greater than or equal to 7.40], the cell's natural Cl(-)HCO3- exchanger mimicked the actions of the tributyltin compound and was the principal factor controlling steady-state pHi. However, with increasing extracellular acidification, there was a progressive deviation from the predicted equilibrium distribution in the case of the normal Cl(-)-HCO3- transport system, whereas tributyltin-treated cells followed theoretical expectations. Exposure of neutrophils to a number of inhibitors of Cl(-)-HCO3- exchange led to a fall in pHi, apparently confirming the impression that a net HCO3- influx through Cl(-)-HCO3- countertransport was chiefly responsible for maintaining steady-state pHi. However, this intracellular acidification could be satisfactorily ascribed to proton movements through a parallel pathway, namely nonionic diffusion of the free acid form of the drugs. These results imply that Cl(-)-HCO3- exchange is the dominant pH regulatory device only under relatively alkaline conditions and that other mechanisms in addition to Na(+)-H+ exchange are likely to play an important role in recovery from acidification and in maintaining steady-state pHi. The possibility that the lactate carrier may function in this capacity is discussed. <\/jats:p>","DOI":"10.1152\/ajpcell.1991.261.5.c906","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T13:18:11Z","timestamp":1514121491000},"page":"C906-C915","source":"Crossref","is-referenced-by-count":13,"title":["Use of tributyltin to probe contribution of Cl(-)-HCO3- exchange to regulation of steady-state pHi in human neutrophils"],"prefix":"10.1152","volume":"261","author":[{"given":"L.","family":"Simchowitz","sequence":"first","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, St. Louis,Missouri."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A.","family":"Textor","sequence":"additional","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, St. Louis,Missouri."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. K.","family":"Vogt","sequence":"additional","affiliation":[{"name":"Department of Medicine, Veterans Affairs Medical Center, St. Louis,Missouri."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1991.261.5.C906","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:46:37Z","timestamp":1718653597000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1991.261.5.C906"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1991,11,1]]}},"alternative-id":["10.1152\/ajpcell.1991.261.5.C906"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1991.261.5.c906","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,11,1]]}}}