{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:48:53Z","timestamp":1762102133846},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1992,2,1]]},"abstract":"<jats:p> Simultaneous measurements of intracellular pH (pHi) and tension development were made in resting cat papillary muscles and resting ventricular myocytes (cat, guinea pig). pH microelectrodes and the fluorescent indicator carboxy-seminaphthorhodafluor-1 (SNARF-1) were used to measure pHi in muscles and myocytes, respectively. pHi-induced changes in isometric muscle tension and myocyte length were elicited by variations in PCO2, HCO3-, and [NH4Cl]. Increased pHi elevated resting tension and decreased resting cell length, whereas decreased pHi decreased tension and increased cell length. The tension-pHi and cell length-pHi relationships were nonlinear and displayed hysteresis. A reduction in extracellular [Ca2+] from 2.7 to 0.5 mM caused little or no change in the tension and cell length responses to elevated pHi. Ca2+ uptake and\/or release by the sarcoplasmic reticulum (SR) does not appear to be involved in the tension response to intracellular alkalosis because the response was unaffected by a postpacing rest period and was not inhibited by ryanodine (5 microM), which depletes SR Ca2+ stores. The cross-bridge inhibitor 2,3-butanedione monoxime (15 mM), but not internal dialysis with 14 mM ethylene glycol-bis(beta-aminoethyl ether)N,N,N',N'-tetraacetic acid, inhibited myocyte contractures elicited by elevated pHi. The latter finding suggests that factors other than pHi-induced increase in myofilament Ca2+ sensitivity may contribute to the resting contractile response to elevated pHi. <\/jats:p>","DOI":"10.1152\/ajpcell.1992.262.2.c316","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T13:06:43Z","timestamp":1514120803000},"page":"C316-C327","source":"Crossref","is-referenced-by-count":29,"title":["Relationship between intracellular pH and tension development in resting ventricular muscle and myocytes"],"prefix":"10.1152","volume":"262","author":[{"given":"K. W.","family":"Spitzer","sequence":"first","affiliation":[{"name":"Nora Eccles Harrison Cardiovascular Research and Training Institute,University of Utah, Salt Lake City 84112."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Bridge","sequence":"additional","affiliation":[{"name":"Nora Eccles Harrison Cardiovascular Research and Training Institute,University of Utah, Salt Lake City 84112."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1992.262.2.C316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:49Z","timestamp":1718653489000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1992.262.2.C316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,2,1]]}},"alternative-id":["10.1152\/ajpcell.1992.262.2.C316"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1992.262.2.c316","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,2,1]]}}}