{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:48:52Z","timestamp":1762102132207},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1992,2,1]]},"abstract":"<jats:p> Anisosmotic cell swelling triggers a regulatory volume decrease (RVD) in cell lines derived from human nonpigmented ciliary epithelium. Measurements of cell volume have indicated that the RVD reflects activation of K+ and\/or Cl- channels. We have begun to characterize the putative channels by whole cell patch clamping. The results obtained by altering the external K+ and Cl- concentrations and by adding 20-50 microM quinidine or 1 mM Ba2+ indicate that K+ conductances contribute substantially and Cl- conductances contribute very little to the total membrane conductance (GT) under baseline isotonic conditions. Reducing the external osmolality by 20-50% reversibly and reproducibly increased GT by an order of magnitude. Data obtained from ion substitutions and the channel blockers quinidine and 5-nitro-2-(3-phenylpropylamino)-benzoate indicate that most of the hypotonicity-induced conductance reflects stationary Cl(-)-channel activity. The contribution of new K(+)-channel activity was small at intracellular free Ca2+ concentrations of 10 or 200 nM. We conclude that the RVD triggered by bath hypotonicity primarily reflects increased Cl(-)-channel activity. <\/jats:p>","DOI":"10.1152\/ajpcell.1992.262.2.c501","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T13:06:43Z","timestamp":1514120803000},"page":"C501-C509","source":"Crossref","is-referenced-by-count":39,"title":["Whole cell patch clamping of ciliary epithelial cells during anisosmotic swelling"],"prefix":"10.1152","volume":"262","author":[{"given":"R. E.","family":"Yantorno","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia19104."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. A.","family":"Carre","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia19104."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Coca-Prados","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia19104."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Krupin","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia19104."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M.","family":"Civan","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Pennsylvania, Philadelphia19104."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1992.262.2.C501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:13Z","timestamp":1718653453000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1992.262.2.C501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,2,1]]}},"alternative-id":["10.1152\/ajpcell.1992.262.2.C501"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1992.262.2.c501","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,2,1]]}}}