{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T18:40:54Z","timestamp":1743100854834},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1992,5,1]]},"abstract":"<jats:p> With the use of the whole cell arrangement of the patch-clamp technique, an outward-directed time-dependent potassium current was identified in cultured chicken growth plate chondrocytes. This delayed rectifier potassium current (IK) activated with a sigmoidal time course during voltage steps to potentials positive to -40 mV. The half-maximal voltage required for current activation was determined to be -8 mV. The reversal potential (Erev) for IK, measured using deactivating tail currents, was -72 mV in the presence of 140 mM internal and 5 mM external [K+] solutions. Changes in external [K+] caused Erev to shift in a manner expected for a potassium-selective channel. In addition, increasing external [K+] from 5 to 50 mM caused the slope conductance of the tail currents to increase twofold. The chondrocyte IK was inhibited by the potassium-channel blocker 4-aminopyridine (4-AP) at concentrations of 0.5-4 mM and by the scorpion venom toxin charybdotoxin (CTX; 10 nM) but was unaffected by 10 mM tetraethylammonium (TEA). Addition of 20 microM ZnCl2 reduced IK in a voltage-dependent manner with the greatest inhibition found to occur at potentials near the threshold for current activation. Reduction of IK by ZnCl2 was accompanied by a slowing in the kinetics of IK activation. On the basis of the gating and pharmacological properties of this current, it is suggested that the chondrocyte channel belongs to a superfamily of K+ channels found in bone and immune system cells. The chondrocyte K+ channel may contribute to the unusually high [K+] found in the extracellular fluid of growth plate cartilage. <\/jats:p>","DOI":"10.1152\/ajpcell.1992.262.5.c1335","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T12:57:58Z","timestamp":1514120278000},"page":"C1335-C1340","source":"Crossref","is-referenced-by-count":20,"title":["Characterization of a delayed rectifier potassium current in chicken growth plate chondrocytes"],"prefix":"10.1152","volume":"262","author":[{"given":"K. B.","family":"Walsh","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of South Carolina School ofMedicine, Columbia 29208."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. D.","family":"Cannon","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of South Carolina School ofMedicine, Columbia 29208."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Wuthier","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of South Carolina School ofMedicine, Columbia 29208."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1992.262.5.C1335","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:32Z","timestamp":1718653472000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1992.262.5.C1335"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,5,1]]}},"alternative-id":["10.1152\/ajpcell.1992.262.5.C1335"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1992.262.5.c1335","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5,1]]}}}