{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:55:10Z","timestamp":1767707710840},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1992,8,1]]},"abstract":"<jats:p> In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2. Furthermore, changes of R depend on the presence of extracellular Ca2+ (Ca2+o) but are much less influenced by changes in extracellular Mg2+ (Mg2+o). To evaluate interference with measurement of cytosolic Mg2+ (Mg2+i) by changes in Ca2+i, we determined the dissociation constant (Kd) and Hill coefficient (NH) of the Ca(2+)-mag-fura-2 and Mg(2+)-mag-fura-2 complexes in standard solutions, in intact acini after loading with the acetoxymethyl ester of mag-fura-2 (mag-fura-2\/AM), and in lysates derived from acini loaded with mag-fura-2\/AM. The Kd of the Ca(2+)-mag-fura-2 complex in acini (determined with ionomycin) was 20.49 +\/- 5.20 microM, and NH was 1.44 +\/- 0.16 (n = 24). Kd of the Mg(2+)-mag-fura-2 complex in acini was 2.25 +\/- 0.98 mM, and NH was 1.20 +\/- 0.20 (n = 25). Mean Kd values were slightly lower in acinar lysates and in solutions of standard mag-fura-2. In acini from either gland, 1,2-bis(2-aminophenoxy)ethan-N,N,N',N'-tetraacetic acid (BAPTA) suppressed carbachol-induced Ca2+i transients. The R value in stimulated acini loaded with BAPTA and mag-fura-2 increased slightly when Mg2+o was increased from less than 10 nM to 1.2 mM, suggesting that Mg2+ influx contributes to the maintenance of Mg2+i during exposure to carbachol. Under these conditions, pancreatic acinar Mg2+i is 0.53 +\/- 0.14 mM (n = 5).(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1992.263.2.c300","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T13:57:42Z","timestamp":1514123862000},"page":"C300-C307","source":"Crossref","is-referenced-by-count":49,"title":["Changes of cytosolic Ca2+ interfere with measurements of cytosolic Mg2+ using mag-fura-2"],"prefix":"10.1152","volume":"263","author":[{"given":"T. W.","family":"Hurley","sequence":"first","affiliation":[{"name":"Department of Child Health, School of Medicine, University ofMissouri-Columbia 65212."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. P.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Department of Child Health, School of Medicine, University ofMissouri-Columbia 65212."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. W.","family":"Brinck","sequence":"additional","affiliation":[{"name":"Department of Child Health, School of Medicine, University ofMissouri-Columbia 65212."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1992.263.2.C300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:48Z","timestamp":1718653488000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1992.263.2.C300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,8,1]]}},"alternative-id":["10.1152\/ajpcell.1992.263.2.C300"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1992.263.2.c300","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,8,1]]}}}