{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:08:05Z","timestamp":1718669285223},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1992,11,1]]},"abstract":"<jats:p> Lymphocytes contain a homogeneous population of beta 2-adrenoceptors. However, their physiological role in the regulation of lymphocyte function, especially early in the activation\/proliferation process, remains unclear. To study the role of beta-adrenergic activation on the regulation of membrane transport, we examined 86Rb uptake in the Jurkat human lymphoma cell line. 86Rb uptake was predominantly accounted for by bumetanide-sensitive uptake (74 +\/- 2% total 86Rb uptake) and ouabain-sensitive uptake (20 +\/- 2%). Bumetanide potently inhibited 86Rb uptake (50% inhibitory concentration = 210 +\/- 40 nM), and bumetanide-sensitive uptake was dependent on extracellular sodium and chloride, consistent with uptake via Na-K-Cl cotransport. The beta-adrenergic agonist isoproterenol (10 microM) mediated a 50 +\/- 11% reduction in bumetanide-sensitive uptake without a significant alteration in ouabain-sensitive uptake. The effect of isoproterenol was potent (50% effective concentration = 4.42 +\/- 1.70 nM), stereoselective, and was inhibited by the beta-adrenergic antagonist nadolol. The effect of isoproterenol was mimicked by permeant adenosine 3',5'-cyclic monophosphate analogues but not by pindolol. These data indicate that beta-adrenoceptor activation decreases 86Rb uptake in lymphocytes via inhibition of Na-K-Cl cotransport. Modulation of this transporter could be one mechanism by which beta-adrenergic agonists regulate lymphocyte function. <\/jats:p>","DOI":"10.1152\/ajpcell.1992.263.5.c1015","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:16:46Z","timestamp":1514125006000},"page":"C1015-C1020","source":"Crossref","is-referenced-by-count":4,"title":["Beta-adrenergic inhibition of Na-K-Cl cotransport in lymphocytes"],"prefix":"10.1152","volume":"263","author":[{"given":"R. D.","family":"Feldman","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Western Ontario, London,Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1992.263.5.C1015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:02Z","timestamp":1718653442000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1992.263.5.C1015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,11,1]]}},"alternative-id":["10.1152\/ajpcell.1992.263.5.C1015"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1992.263.5.c1015","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11,1]]}}}