{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:35:51Z","timestamp":1758926151149},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1992,11,1]]},"abstract":"<jats:p> Activation of protein kinase C (PKC) modulates the mobilization of intracellular Ca2+ induced by extracellular ATP in rat ventricular myocytes. Pretreatment of myocytes with PKC activators attenuated both the ATP-induced Ca2+ transient and the noradrenergic potentiation of the Ca2+ response. Various PKC activators decreased both the basal cAMP level and the cAMP levels that had been elevated by norepinephrine, forskolin, or 3-isobutyl-1-methylxanthine. The inhibitory effects of PKC activators were reversed by the PKC inhibitor staurosporine. The ATP-induced Ca2+ response is an integrated response resulting from ATP eliciting an inward cation current (IATP), cellular depolarization, Ca2+ influx through Ca2+ channels, and Ca2+ release from the sarcoplasmic reticulum. We used the whole cell voltage-clamp technique to investigate which steps of this integrated response are affected by PKC. PKC activators did not significantly affect the IATP. In contrast, PKC activators decreased the basal Ca2+ current (ICa) or Ba2+ current and the beta-adrenergic-stimulated ICa. These results suggest that PKC-induced suppression of the ATP-induced Ca2+ response and the beta-adrenergic-potentiated Ca2+ response is achieved at least partially by decreasing the intracellular cAMP level and ICa. <\/jats:p>","DOI":"10.1152\/ajpcell.1992.263.5.c933","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:16:46Z","timestamp":1514125006000},"page":"C933-C940","source":"Crossref","is-referenced-by-count":24,"title":["Ca2+ mobilization by extracellular ATP in rat cardiac myocytes: regulation by protein kinase C and A"],"prefix":"10.1152","volume":"263","author":[{"given":"J. S.","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, School of Medicine, CaseWestern Reserve University, Cleveland, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Christie","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, School of Medicine, CaseWestern Reserve University, Cleveland, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. N.","family":"Levy","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, School of Medicine, CaseWestern Reserve University, Cleveland, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Scarpa","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, School of Medicine, CaseWestern Reserve University, Cleveland, Ohio 44106."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1992.263.5.C933","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:44:13Z","timestamp":1718653453000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1992.263.5.C933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1992,11,1]]}},"alternative-id":["10.1152\/ajpcell.1992.263.5.C933"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1992.263.5.c933","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11,1]]}}}