{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:07:15Z","timestamp":1718669235293},"reference-count":0,"publisher":"American Physiological Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:p> We have examined the mineralocorticoid specificity in a TBM 18-23 cell line derived from the toad bladder epithelium. In cells grown on porous substrate, corticosterone was more potent than aldosterone in stimulating a sodium transport response, measured by the short-circuit current method 6 h after hormone addition [mean affinity constant (K0.5) for corticosterone = 1 nM vs. K0.5 for aldosterone = 8 nM]. The time course of effects and saturation kinetics were identical for both agonists, suggesting interaction with identical receptors. Whereas the dose-response relationship for aldosterone did not change with time of incubation (6 vs. 24 h), the dose-response curve for corticosterone became biphasic at 24-h incubation (apparent K0.5 as high as 40 nM), demonstrating that corticosterone became apparently less potent with time. Pretreatment with carbenoxolone, a potent inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), restored full sensitivity at 24-h incubation to corticosterone. The 11 beta-HSD activity was low during the first 3 h of incubation in the presence of 3 nM corticosterone, and only a small fraction (approximately 7%) of corticosterone was metabolized. At 24-h incubation, 11 beta-HSD activity increased approximately 2.5-fold (P &lt; 0.001, n = 8). We conclude that 11 beta-HSD activity is induced by its own substrate in TBM cells in parallel with the induction of the carbenoxolone-sensitive sodium transport response. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.264.2.c317","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:06:14Z","timestamp":1514124374000},"page":"C317-C322","source":"Crossref","is-referenced-by-count":14,"title":["Corticosterone induces 11 beta-HSD and mineralocorticoid specificity in an amphibian urinary bladder cell line"],"prefix":"10.1152","volume":"264","author":[{"given":"H. P.","family":"Gaeggeler","sequence":"first","affiliation":[{"name":"Institut de Pharmacologie et de Toxicologie de l'Universite, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Duperrex","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Toxicologie de l'Universite, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Hautier","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Toxicologie de l'Universite, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. C.","family":"Rossier","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie et de Toxicologie de l'Universite, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.264.2.C317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:56Z","timestamp":1718653376000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.264.2.C317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2,1]]}},"alternative-id":["10.1152\/ajpcell.1993.264.2.C317"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.264.2.c317","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2,1]]}}}