{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:22:52Z","timestamp":1760955772804},"reference-count":0,"publisher":"American Physiological Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,3,1]]},"abstract":"<jats:p> The adrenergic agonist clonidine activates NaCl cotransport in rabbit tracheocytes. With the use of the high-affinity analogue p-[125I]iodoclonidine, binding of clonidine to cells was determined to fit a two-site model, with one site of high specificity for alpha 2-adrenergic (alpha 2-AR) and the other with a high affinity for I1-imidazol(in)e (I1) receptors. Total density of binding sites for both receptors was similar at 18 fmol\/mg protein. Moxonidine displayed a 166-fold greater specificity for I1 receptors compared with cimetidine. Bumetanide-sensitive Na or Cl transport was stimulated by the alpha 2-AR agonists clonidine or guanabenz but not by the I1 agents cimetidine or moxonidine. I1 agonists-stimulated Na transport was detected only in the presence of bumetanide. Prazosin did not block clonidine-stimulated NaCl uptake or efflux, indicating the presence of an alpha 2A-AR subtype. Addition of clonidine either before or after incubation with l-isoproterenol or forskolin did not attenuate the time- and dose-dependent increase in adenosine 3',5'-cyclic monophosphate (cAMP) levels. Thus clonidine stimulates NaCl cotransport in rabbit tracheocytes through an alpha 2A-AR mechanism that does not require cAMP for signal transduction. In addition, I1-imidazol(in)e receptors stimulate Na transport in rabbit tracheocytes through an unidentified pathway. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.264.3.c568","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T13:27:05Z","timestamp":1514122025000},"page":"C568-C576","source":"Crossref","is-referenced-by-count":17,"title":["Alpha 2-adrenergic, but not imidazole, agonists activate NaCl cotransport in rabbit tracheal epithelial cells"],"prefix":"10.1152","volume":"264","author":[{"given":"C. M.","family":"Liedtke","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Rainbow Babies and Children Hospital,Cleveland, Ohio."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Furin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Rainbow Babies and Children Hospital,Cleveland, Ohio."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Ernsberger","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Rainbow Babies and Children Hospital,Cleveland, Ohio."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.264.3.C568","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:41:19Z","timestamp":1718653279000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.264.3.C568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,3,1]]}},"alternative-id":["10.1152\/ajpcell.1993.264.3.C568"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.264.3.c568","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,3,1]]}}}