{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:28:08Z","timestamp":1761060488007},"reference-count":0,"publisher":"American Physiological Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,4,1]]},"abstract":"<jats:p> The involvement of K+ on the volume regulatory process in astrocytes was investigated by characterizing the hyposmolarity-induced efflux of K+ using 86Rb as a tracer. About 70 and 30% of the intracellular content of 86Rb was released after reductions in osmolarity from 320 to 160 or 220 mosM, respectively, during the time in which cells exhibit a volume regulatory response subsequent to swelling. No significant increase in 86Rb efflux was observed with lower reductions in osmolarity. The 86Rb efflux was Ca2+ independent and insensitive to temperature. It was inhibited by furosemide but not by bumetanide and was unaffected when nitrate, but not gluconate, replaced intracellular Cl-. The efflux was markedly inhibited by quinidine and by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. Quinidine also prevented the volume regulatory decrease of cells, and this effect was overcome when a large cation permeability was imposed by gramicidin. In isosmotic conditions 86Rb efflux was not activated by N-ethylmaleimide, but this drug strongly inhibited the hyposmolarity-activated release. These findings suggest that 86Rb efflux from astrocytes associated to cell swelling is not mediated by an electroneutral cotransporter and rather favor the implication of a conductive exit pathway that may be a Ca(2+)-independent K+ channel. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.264.4.c836","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:00:44Z","timestamp":1514124044000},"page":"C836-C842","source":"Crossref","is-referenced-by-count":25,"title":["Volume-activated Rb+ transport in astrocytes in culture"],"prefix":"10.1152","volume":"264","author":[{"given":"R.","family":"Sanchez-Olea","sequence":"first","affiliation":[{"name":"Institute of Cell Physiology, Universidad Nacional Autonoma de Mexico, Mexico City."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Moran","sequence":"additional","affiliation":[{"name":"Institute of Cell Physiology, Universidad Nacional Autonoma de Mexico, Mexico City."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Institute of Cell Physiology, Universidad Nacional Autonoma de Mexico, Mexico City."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Pasantes-Morales","sequence":"additional","affiliation":[{"name":"Institute of Cell Physiology, Universidad Nacional Autonoma de Mexico, Mexico City."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.264.4.C836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:41:48Z","timestamp":1718653308000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.264.4.C836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,4,1]]}},"alternative-id":["10.1152\/ajpcell.1993.264.4.C836"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.264.4.c836","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4,1]]}}}