{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:34:39Z","timestamp":1773300879919,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,5,1]]},"abstract":"<jats:p> Patch-clamp recordings from rabbit corneal epithelial cells have identified a large-conductance (167 pS in symmetrical 150 mM KCl) K channel that is the major contributor to the whole cell current (J. L. Rae and G. Farrugia. J. Membr. Biol. 129: 81-87, 1992). We report here on the regulation of this channel by changes in cellular osmolality and\/or volume. Exchanging the bath solution with a hyposmotic (225 or 150 mosM) solution resulted in cellular swelling and selective activation of the K current (126 +\/- 86 and 273 +\/- 184% increase, respectively). Hyperosmotic solution changes (380 mosM) resulted in cell shrinkage and deactivation of the K current (44.2 +\/- 21% decrease). Similar increases in the cell volume and whole cell current were observed on increasing (in perforated patch experiments) the chloride ion concentration (50 mM) in the pipette intracellular solution (127 +\/- 63% increase). These changes were accompanied by marked shifts in the resting membrane voltage. We conclude that the K channels in these cells can respond to alteration in cellular osmolality or volume, resulting in changes in the whole cell current and resting voltage. <\/jats:p>","DOI":"10.1152\/ajpcell.1993.264.5.c1238","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T14:53:56Z","timestamp":1514127236000},"page":"C1238-C1245","source":"Crossref","is-referenced-by-count":30,"title":["Effect of volume changes on a potassium current in rabbit corneal epithelial cells"],"prefix":"10.1152","volume":"264","author":[{"given":"G.","family":"Farrugia","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, Mayo Foundation, Rochester,Minnesota 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Rae","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Mayo Foundation, Rochester,Minnesota 55905."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.264.5.C1238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:42:16Z","timestamp":1718653336000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.264.5.C1238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,5,1]]}},"alternative-id":["10.1152\/ajpcell.1993.264.5.C1238"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.264.5.c1238","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5,1]]}}}