{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T17:36:54Z","timestamp":1770831414552,"version":"3.50.1"},"reference-count":0,"publisher":"American Physiological Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Physiology-Cell Physiology"],"published-print":{"date-parts":[[1993,6,1]]},"abstract":"<jats:p> The basal levels of inositol monophosphate, inositol bisphosphate, and inositol trisphosphate (InsP3) in A-431 cells incubated in Na(+)-Hanks' solution were, respectively, 1.23 +\/- 0.18, 0.17 +\/- 0.03, and 0.69 +\/- 0.07% of the total radioactivity in the cell. When cells were heated, InsP3 increased in a temperature-dependent manner related to the duration of heating. The active form of InsP3, inositol 1,4,5-trisphosphate, increased 237 +\/- 17% after heating (45 degrees C, 20 min) then returned to baseline within 15 min after the return to 37 degrees C. The heat-induced increase in InsP3 was not observed in the absence of extracellular Ca2+ or with amiloride treatment. Treatment with the nonhydrolyzable GTP analogue 5'-guanylylimidodiphosphate stimulated that component of the InsP3 increase due to G proteins. U-73122, an inhibitor of phospholipase C-mediated processes, blocked the increase in InsP3 resulting from heat exposure. Both pertussis toxin (30 ng\/ml, 24 h), an inhibitor of G inhibitory protein, and cholera toxin (1 microgram\/ml, 1 h), a stimulator of G stimulatory protein, increased InsP3 in unheated cells, and heating failed to induce a further increase, suggesting that heat activates G proteins. Likewise, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP), 3-isobutyl-1-methylxanthine, Ro 20-1724, or forskolin increased InsP3 in unheated cells, and heat did not cause an additional increase. The InsP3 increase induced by 8-BrcAMP was inhibited by removal of extracellular Ca2+ or treatment with verapamil, suggesting that an influx of extracellular Ca2+ stimulates InsP3 production.(ABSTRACT TRUNCATED AT 250 WORDS) <\/jats:p>","DOI":"10.1152\/ajpcell.1993.264.6.c1561","type":"journal-article","created":{"date-parts":[[2017,12,24]],"date-time":"2017-12-24T15:03:34Z","timestamp":1514127814000},"page":"C1561-C1569","source":"Crossref","is-referenced-by-count":24,"title":["Effect of heat shock, [Ca2+]i, and cAMP on inositol trisphosphate in human epidermoid A-431 cells"],"prefix":"10.1152","volume":"264","author":[{"given":"J. G.","family":"Kiang","sequence":"first","affiliation":[{"name":"Department of Clinical Physiology, Walter Reed Army Institute ofResearch, Washington, District of Columbia 20307-5100."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. E.","family":"McClain","sequence":"additional","affiliation":[{"name":"Department of Clinical Physiology, Walter Reed Army Institute ofResearch, Washington, District of Columbia 20307-5100."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"24","container-title":["American Journal of Physiology-Cell Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.physiology.org\/doi\/pdf\/10.1152\/ajpcell.1993.264.6.C1561","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:41:37Z","timestamp":1718653297000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.physiology.org\/doi\/10.1152\/ajpcell.1993.264.6.C1561"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1993,6,1]]}},"alternative-id":["10.1152\/ajpcell.1993.264.6.C1561"],"URL":"https:\/\/doi.org\/10.1152\/ajpcell.1993.264.6.c1561","relation":{},"ISSN":["0363-6143","1522-1563"],"issn-type":[{"value":"0363-6143","type":"print"},{"value":"1522-1563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,6,1]]}}}